Document Detail


DNA affinity column chromatography: application in the isolation of distinct antibody populations from SLE sera.
MedLine Citation:
PMID:  3878747     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An improved method was developed for purification of anti-DNA antibodies by single-stranded DNA (ssDNA), or double-stranded DNA (dsDNA) affinity column chromatography. The effectiveness of this method was examined by measuring the recoveries and purifications of various monoclonal and polyclonal antibodies. Results showed that this method gave good recoveries and purifications of anti-DNA antibodies. Furthermore, by this method, it was possible to isolate antibody populations with distinct specificities from polyclonal antibodies, as shown by enzyme linked immunosorbent assay (ELISA). Therefore, this method should be useful for characterization of polyclonal antibodies in the sera of patients with systemic lupus erythematosus (SLE) as well as of autoimmune lupus mice.
Authors:
T Kubota; T Akatsuka; Y Kanai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  62     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  1985 Nov 
Date Detail:
Created Date:  1986-03-12     Completed Date:  1986-03-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  321-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Antinuclear / isolation & purification*
Antibody Affinity
Chromatography, Affinity
Cross Reactions
DNA / immunology
DNA, Single-Stranded / immunology
Enzyme-Linked Immunosorbent Assay
Humans
Lupus Erythematosus, Systemic / immunology*
Mice
Mice, Inbred Strains
Chemical
Reg. No./Substance:
0/Antibodies, Antinuclear; 0/DNA, Single-Stranded; 9007-49-2/DNA
Comments/Corrections

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