| DNA affinity column chromatography: application in the isolation of distinct antibody populations from SLE sera. | |
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MedLine Citation:
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PMID: 3878747 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An improved method was developed for purification of anti-DNA antibodies by single-stranded DNA (ssDNA), or double-stranded DNA (dsDNA) affinity column chromatography. The effectiveness of this method was examined by measuring the recoveries and purifications of various monoclonal and polyclonal antibodies. Results showed that this method gave good recoveries and purifications of anti-DNA antibodies. Furthermore, by this method, it was possible to isolate antibody populations with distinct specificities from polyclonal antibodies, as shown by enzyme linked immunosorbent assay (ELISA). Therefore, this method should be useful for characterization of polyclonal antibodies in the sera of patients with systemic lupus erythematosus (SLE) as well as of autoimmune lupus mice. |
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Authors:
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T Kubota; T Akatsuka; Y Kanai |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical and experimental immunology Volume: 62 ISSN: 0009-9104 ISO Abbreviation: Clin. Exp. Immunol. Publication Date: 1985 Nov |
Date Detail:
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Created Date: 1986-03-12 Completed Date: 1986-03-12 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0057202 Medline TA: Clin Exp Immunol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 321-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Antinuclear / isolation & purification* Antibody Affinity Chromatography, Affinity Cross Reactions DNA / immunology DNA, Single-Stranded / immunology Enzyme-Linked Immunosorbent Assay Humans Lupus Erythematosus, Systemic / immunology* Mice Mice, Inbred Strains |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Antinuclear; 0/DNA, Single-Stranded; 9007-49-2/DNA |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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