| DNA Index in childhood acute lymphoblastic leukaemia: a karyotypic method to validate the flow cytometric measurement. | |
| | |
MedLine Citation:
|
PMID: 19793113 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The DNA index (DI) is a prognostic factor in childhood acute lymphoblastic leukemia (ALL). The accuracy of DI measurement is important for treatment stratification: hyperdiploidy with DI > or = 1.16 is predictive of favorable prognosis whereas hypodiploidy is associated with poor prognosis. The aim of this study was to validate the accuracy of the DI measured by flow cytometry (FCM) by comparison with the karyotype. From samples of 112 childhood ALL, we created a formula to calculate a theoretical DNA index (tDI) based on the blast cell karyotype, taking into account the additional or missing chromosome material of the major clone. FCM DI correlated with tDI calculated from karyotype (R = 0.987) and with modal chromosome number (DI = 0.0202 x Modal NB + 0.0675 and R = 0.984). In three cases a hypodiploid blast cell population was detected by FCM, while only the duplicated clone was identified by the karyotype. The strong correlation between tDI and DI validates the accuracy of FCM quantification, which is technically fast on fresh or frozen samples. If the karyotype is essential to analyze chromosomal abnormalities, FCM provides complementary information in aneuploid ALLs, either by confirming the cytogenetic data or by detecting additional clones not identified when only using cytogenetic data. |
| | |
Authors:
|
P Rachieru-Sourisseau; L Baranger; N Dastugue; A Robert; F Geneviève; E Kuhlein; A Chassevent |
Related Documents
:
|
3086723 - Differentiation of leukemia cells to polymorphonuclear leukocytes in patients with acut... 10720153 - Molecular genetics in acute leukemia. 17551333 - Velocardiofacial syndrome with a rare t(2;22). |
Publication Detail:
|
Type: Journal Article; Validation Studies Date: 2009-09-28 |
Journal Detail:
|
Title: International journal of laboratory hematology Volume: 32 ISSN: 1751-553X ISO Abbreviation: Int J Lab Hematol Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-06-11 Completed Date: 2010-09-23 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101300213 Medline TA: Int J Lab Hematol Country: England |
Other Details:
|
Languages: eng Pagination: 288-98 Citation Subset: IM |
Affiliation:
|
Unité d'Hématologie-Immunologie-Oncologie, Pole Enfant, CHU Angers, France. PeRachieru@chu-angers.fr |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adolescent B-Lymphocytes / immunology Cell Lineage Child Child, Preschool DNA / analysis* Female Flow Cytometry / methods* Humans Infant Karyotyping / methods* Male Ploidies Precursor Cell Lymphoblastic Leukemia-Lymphoma / diagnosis*, genetics Prognosis T-Lymphocytes / immunology |
| Chemical | |
Reg. No./Substance:
|
9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Utility of hematologic and volume, conductivity, and scatter parameters from umbilical cord blood in...
Next Document: Parametric quantification of myocardial ischaemia using real-time perfusion adenosine stress echocar...