| DNA Hypermethylation in Somatic Cells Correlates with Higher Reprogramming Efficiency. | |
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MedLine Citation:
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PMID: 22653871 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The efficiency of somatic cell reprogramming to pluripotency using defined factors is dramatically affected by the cell type of origin. Here we show that human keratinocytes, which can be reprogrammed at a higher efficiency than fibroblast(1) , share more genes hypermethylated at CpGs with human ES cells than other somatic cells frequently used for reprogramming. Moreover, pluripotent cells reprogrammed from keratinocytes (KiPS) are more similar to ES cells that those reprogrammed from fibroblasts (FiPS) in regards to DNA methylation levels, mostly due to the presence of genes that fail to acquire high levels of DNA methylation in FiPS cells. We propose that higher reprogramming efficiency correlates with the hypermethylation of tissue specific genes, rather than with a more permissive pluripotency gene network. |
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Authors:
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María J Barrero; María Berdasco; Ida Paramonov; Josipa Bilic; Marianna Vitaloni; Manel Esteller; Juan Carlos Izpisua Belmonte |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-31 |
Journal Detail:
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Title: Stem cells (Dayton, Ohio) Volume: - ISSN: 1549-4918 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-6-1 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9304532 Medline TA: Stem Cells Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 AlphaMed Press. |
Affiliation:
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Center for Regenerative Medicine in Barcelona, Doctor Aiguader 88, E-08003, Barcelona, Catalonia, Spain. |
Export Citation:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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