Document Detail


DNA hypermethylation in somatic cells correlates with higher reprogramming efficiency.
MedLine Citation:
PMID:  22653871     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The efficiency of somatic cell reprogramming to pluripotency using defined factors is dramatically affected by the cell type of origin. Here, we show that human keratinocytes, which can be reprogrammed at a higher efficiency than fibroblast [Nat Biotechnol 2008;26:1276-1284], share more genes hypermethylated at CpGs with human embryonic stem cells (ESCs) than other somatic cells frequently used for reprogramming. Moreover, pluripotent cells reprogrammed from keratinocytes (KiPS) are more similar to ESCs than those reprogrammed from fibroblasts (FiPS) in regard to DNA methylation levels, mostly due to the presence of genes that fail to acquire high levels of DNA methylation in FiPS cells. We propose that higher reprogramming efficiency correlates with the hypermethylation of tissue-specific genes rather than with a more permissive pluripotency gene network.
Authors:
María J Barrero; María Berdasco; Ida Paramonov; Josipa Bilic; Marianna Vitaloni; Manel Esteller; Juan Carlos Izpisua Belmonte
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  30     ISSN:  1549-4918     ISO Abbreviation:  Stem Cells     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-25     Completed Date:  2013-02-28     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1696-702     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 AlphaMed Press.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques
Cell Differentiation / genetics
DNA Methylation*
Epigenesis, Genetic
Female
Fibroblasts / metabolism,  physiology
Gene Expression Profiling
Humans
Induced Pluripotent Stem Cells / metabolism,  physiology*
Male
Mice
Nuclear Reprogramming / genetics*
Pluripotent Stem Cells / metabolism,  physiology
Grant Support
ID/Acronym/Agency:
268626//European Research Council; P30 CA014195/CA/NCI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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