Document Detail


DNA extraction from freeze-dried plant tissue with CTAB in a 96-well format.
MedLine Citation:
PMID:  21041389     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
INTRODUCTION This protocol is a modified version of DNA isolation using cetyltrimethylammonium bromide (CTAB) and 96-well plates. It is high-throughput, which facilitates the analysis of large mapping populations. The DNA yield is adequate for at least 100-500 polymerase chain reaction (PCR) procedures.
Authors:
An-Ping Hsia; Hsin D Chen; Kazuhiro Ohtsu; Patrick S Schnable
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Publication Detail:
Type:  Journal Article     Date:  2010-11-01
Journal Detail:
Title:  Cold Spring Harbor protocols     Volume:  2010     ISSN:  1559-6095     ISO Abbreviation:  Cold Spring Harb Protoc     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101524530     Medline TA:  Cold Spring Harb Protoc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  pdb.prot5516     Citation Subset:  IM    
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