Document Detail


DNA damage enhancement from gold nanoparticles for clinical MV photon beams.
MedLine Citation:
PMID:  23148509     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we quantify the relative damage enhancement due to the presence of gold nanoparticles (GNP) in vitro in a clinical 6 MV beam for various delivery parameters and depths. It is expected that depths and delivery modes that produce a larger proportions of low-energy photons will have a larger effect on the cell samples containing GNP. HeLa cells with and without 50 nm GNP were irradiated at depths of 1.5, 5, 10, 15 and 20 cm. Conventional beams with square aperture sizes 5, 10 and 15 cm at isocenter, and flattening filter free (FFF) beams were used. Relative DNA damage enhancement with GNP was evaluated by γ-H2AX staining. Statistically significant increases in DNA damage with GNP, compared to the absence of GNP, were observed for all depths and delivery modes. Relative to the shallowest depth, damage enhancement was observed to increase as a function of increasing depth for all deliveries. For the conventional (open field) delivery, DNA damage enhancement with GNP was seen to increase as a function of field size. For FFF delivery, a substantial increase in enhancement was found relative to the conventional field delivery. The measured relative DNA damage enhancement validates the theoretically predicted trends as a function of depth and delivery mode for clinical MV photon beams. The results of this study open new possibilities for the clinical development of gold nanoparticle-aided radiation therapy.
Authors:
Ross I Berbeco; Houari Korideck; Wilfred Ngwa; Rajiv Kumar; Janki Patel; Srinivas Sridhar; Sarah Johnson; Brendan D Price; Alec Kimmelman; G Mike Makrigiorgos
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-11-13
Journal Detail:
Title:  Radiation research     Volume:  178     ISSN:  1938-5404     ISO Abbreviation:  Radiat. Res.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-12     Completed Date:  2013-02-06     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  604-8     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
DNA Damage*
Gold / chemistry*,  pharmacology*
HeLa Cells
Humans
Metal Nanoparticles*
Photons / therapeutic use*
Grant Support
ID/Acronym/Agency:
CA93602/CA/NCI NIH HHS; R01 CA064585/CA/NCI NIH HHS; R01 CA093602/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
7440-57-5/Gold
Comments/Corrections

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