Document Detail


DNA-Based Identification of Fish Species Implicated in Puffer Fish Poisoning.
MedLine Citation:
PMID:  22200802     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A method for identification of fish species using three different mitochondrial DNA regions, 16S rRNA, cytochrome b and cytochrome c gene fragments, was investigated. The combined use of all three regions enabled reliable species identification in not only raw fish, but also dried, seasoned and boiled fish, products. Furthermore, the method was applicable even to vomitus from a patient involved in a puffer fish poisoning incident. However, further improvement is necessary to discriminate between closely related species such as Takifugu rubripes and T. chinensis, because they showed close similarity in the nucleotide sequences in the three gene fragments analyzed in this study.
Authors:
Taro Murakami; Atsushi Masayama; Masami Ki; Tetsuo Yamano; Mituru Simizu
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan     Volume:  52     ISSN:  1882-1006     ISO Abbreviation:  Shokuhin Eiseigaku Zasshi     Publication Date:  2011  
Date Detail:
Created Date:  2011-12-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0142214     Medline TA:  Shokuhin Eiseigaku Zasshi     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  348-53     Citation Subset:  IM    
Affiliation:
Osaka City Institute of Public Health and Environmental Sciences.
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