Document Detail


DNA-Based Diagnostics for Genetically Modified Cotton: Decaplex PCR Assay to Differentiate MON531 and MON15985 Bt Cotton Events.
MedLine Citation:
PMID:  23143489     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The adoption rate and global area under cultivation of genetically modified (GM) crops is dramatically increasing in recent past. GM cotton has occupied 25.0 million hectares (mha) comprising 15.6% of the global area under GM cultivation. Bt cotton, expressing delta-endotoxins from Bacillus thuringiensis (Bt), is the only commercialized crop in India that is planted on an area of 10.6 mha. With the increase in development and commercialization of GM crops, it is necessary to develop appropriate qualitative and quantitative methods for detection of different GM events. Robust diagnostics for GM detection need to be developed and implemented to monitor and detect different events of GM cotton in India. This chapter summarizes the methods based on polymerase chain reaction (PCR) being employed for detection of different GM events of cotton. We describe a decaplex PCR method for identification and differentiation of two major commercialized events of Bt cotton, i.e., MON531 and MON15985, in India.
Authors:
Gurinder Jit Randhawa; Monika Singh; Rashmi Chhabra
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  958     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  139-51     Citation Subset:  IM    
Affiliation:
National Research Centre on DNA Fingerprinting, National Bureau of Plant Genetic Resources, New Delhi, India, gurinder.randhawa@rediffmail.com.
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