Document Detail

DMSO regulates osteoclast development in vitro.
MedLine Citation:
PMID:  21359822     Owner:  NLM     Status:  MEDLINE    
Dimethyl sulfoxide (DMSO) is routinely used in the laboratory as a solvent and vehicle for organic molecules. Although it has been used in previous studies involving myeloid cells and macrophages, we are unaware of data demonstrating the effects of DMSO alone on osteoclast development. Recently, we were using DMSO as a vehicle and included a non-vehicle control. Surprisingly, we observed a marked change in osteoclast development, and therefore designed this study to examine the effects of DMSO on osteoclast development. Osteoclasts were generated from two sources: bone marrow macrophages and an osteoclast progenitor cell line. Cells were cultured with DMSO for various durations and at differing concentrations and mature, multinucleated (>3 nuclei) TRAP(+) cells were assessed in terms of cell number, cell surface area, and number of nuclei/cell. Osteoclast surface area increased in 5 μM DMSO to a mean of 156,422 pixels from a mean of 38,510 pixels in control culture, and subsequently decreased in 10 μM DMSO to a mean of 18,994 pixels. With serial addition of DMSO over 5 d, a significant increase in mean surface area, and number of nuclei/cell was also observed, while the opposite was true when DMSO was serially removed from culture. These findings show that DMSO exerts a marked effect on osteoclast differentiation. Since many investigators use DMSO to solubilize compounds for treatment of osteoclasts, caution is warranted as altering DMSO concentrations may have a profound effect on the final data, especially if osteoclast differentiation is being assessed.
Justin M Lemieux; Gary Wu; Joseph A Morgan; Melissa A Kacena
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-02-27
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  47     ISSN:  1543-706X     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-28     Completed Date:  2011-09-26     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  260-7     Citation Subset:  IM    
Department of Orthopaedics and Rehabilitation, Yale University School of Medicine, New Haven, CT, USA.
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MeSH Terms
Cell Count
Dimethyl Sulfoxide / pharmacology*
Osteoclasts / cytology*,  drug effects*
Surface Properties / drug effects
Time Factors
Grant Support
Reg. No./Substance:
67-68-5/Dimethyl Sulfoxide

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