Document Detail

DMBA induces programmed cell death (apoptosis) in the A20.1 murine B cell lymphoma.
MedLine Citation:
PMID:  8365579     Owner:  NLM     Status:  MEDLINE    
The mechanism by which 7,12-dimethylbenz[a]anthracene (DMBA) produces cytotoxicity in lymphocytes was investigated in these studies using the murine A20.1 B cell lymphoma. Results show that in vitro exposure of these cells to 10-30 microM DMBA for 4 hr produced an increase in intracellular Ca2+, DNA fragmentation, and subsequent cell death. Elevation of Ca2+ and DNA fragmentation induced by DMBA were greatly pronounced when the A20.1 cells were exposed at high cell density (10(7) cells/ml). DMBA-induced DNA fragmentation and cell death were inhibited by coexposure of A20.1 cells to a calcium chelator (EDTA), a general nuclease and polymerase inhibitor (aurintricarboxylic acid), and a protein synthesis inhibitor (cycloheximide). These agents have been previously shown to inhibit apoptosis in lymphocytes and other cells exposed to chemical agents. We also found that cyclosporin A, an inhibitor of Ca(2+)-dependent pathways of T and B cell activation, prevented apoptosis in the A20.1 cell line. These results demonstrate that DMBA induces programmed cell death (apoptosis) in the A20.1 murine B cell lymphoma by Ca(2+)-dependent pathways. The increased sensitivity of A20.1 at high cell density to Ca2+ elevation and DNA fragmentation suggests that cell to cell interactions may also be important in this process.
S W Burchiel; D A Davis; S D Ray; S L Barton
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Fundamental and applied toxicology : official journal of the Society of Toxicology     Volume:  21     ISSN:  0272-0590     ISO Abbreviation:  Fundam Appl Toxicol     Publication Date:  1993 Jul 
Date Detail:
Created Date:  1993-10-05     Completed Date:  1993-10-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8200838     Medline TA:  Fundam Appl Toxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  120-4     Citation Subset:  IM    
University of New Mexico College of Pharmacy, Toxicology Program, Albuquerque 87131.
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MeSH Terms
9,10-Dimethyl-1,2-benzanthracene / toxicity*
Apoptosis / drug effects*
B-Lymphocytes / drug effects*
Cell Division / drug effects
DNA, Neoplasm / drug effects
Lymphoma, B-Cell
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/DNA, Neoplasm; 57-97-6/9,10-Dimethyl-1,2-benzanthracene

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