Document Detail

DIPI and DAPI: fluorescence banding with only negliglible fading.
MedLine Citation:
PMID:  67075     Owner:  NLM     Status:  MEDLINE    
DIPI and DAPI produce distinct fluorescent bands in human chromosomes similar to quinacrine banding patterns. Additionally, the AT rich secondary constrictions in the chromosomes Nos. 1, 9 and 16 are brightly fluorescent. On the other hand the brilliantly fluorescent regions after staining with quinacrine mustard in the chromosomes Nos. 3 and 4, satellites and some other regions in the acrocentric chromosomes are less striking. The distal part of the Y, however, is clearly discernible. Thus DIPI and DAPI seem to be strictly AT specific fluorochromes like Hoechst 33258. In interphase nuclei the Y chromosome can be identified. However, quinacrines are superior for Y-body analysis in buccal, hair cell and sperm smears. BrdU labeled chromatids show reduced fluorescence intensity. The difference, however, is less apparent than after staining with Hoechst 33 258. DAPI and especially DIPI are highly resistant to UV-irradiation; there is almost no fading within 30 min when using DIPI. Moreover, fluorescence intensity is stronger than in quinicrines. When photographing, exposure times may be reduced to about one quarter compared to quinacrine mustard.
W Schnedl; A V Mikelsaar; M Breitenbach; O Dann
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Human genetics     Volume:  36     ISSN:  0340-6717     ISO Abbreviation:  Hum. Genet.     Publication Date:  1977 Apr 
Date Detail:
Created Date:  1977-06-30     Completed Date:  1977-06-30     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7613873     Medline TA:  Hum Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  167-72     Citation Subset:  IM    
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MeSH Terms
Amidines / diagnostic use
Chromosomes / analysis*
Fluorescent Dyes*
Imidazoles / diagnostic use*
Indoles / diagnostic use*
Staining and Labeling
Ultraviolet Rays
Reg. No./Substance:
0/Amidines; 0/Fluorescent Dyes; 0/Imidazoles; 0/Indoles

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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