Document Detail


A DIGE proteomic analysis for high-intensity exercise-trained rat skeletal muscle.
MedLine Citation:
PMID:  20634418     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Exercise training induces various adaptations in skeletal muscles. However, the mechanisms remain unclear. In this study, we conducted 2D-DIGE proteomic analysis, which has not yet been used for elucidating adaptations of skeletal muscle after high-intensity exercise training (HIT). For 5 days, rats performed HIT, which consisted of 14 20-s swimming exercise bouts carrying a weight (14% of the body weight), and 10-s pause between bouts. The 2D-DIGE analysis was conducted on epitrochlearis muscles excised 18 h after the final training exercise. Proteomic profiling revealed that out of 800 detected and matched spots, 13 proteins exhibited changed expression by HIT compared with sedentary rats. All proteins were identified by MALDI-TOF/MS. Furthermore, using western immunoblot analyses, significantly changed expressions of NDUFS1 and parvalbumin (PV) were validated in relation to HIT. In conclusion, the proteomic 2D-DIGE analysis following HIT-identified expressions of NDUFS1 and PV, previously unknown to have functions related to exercise-training adaptations.
Authors:
Wataru Yamaguchi; Eri Fujimoto; Mitsuru Higuchi; Izumi Tabata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-15
Journal Detail:
Title:  Journal of biochemistry     Volume:  148     ISSN:  1756-2651     ISO Abbreviation:  J. Biochem.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-31     Completed Date:  2011-01-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  327-33     Citation Subset:  IM    
Affiliation:
Health Promotion and Exercise Program, National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku, Tokyo 162-8636, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adaptation, Physiological*
Animals
Gene Expression Regulation / physiology
Muscle Proteins / analysis*
Muscle, Skeletal*
NADH Dehydrogenase / analysis
Parvalbumins / analysis
Physical Conditioning, Animal*
Proteomics / methods*
Rats
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Swimming
Two-Dimensional Difference Gel Electrophoresis
Chemical
Reg. No./Substance:
0/Muscle Proteins; 0/Parvalbumins; EC 1.6.99.3/NADH Dehydrogenase

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