Document Detail


DHEA-Bodipy--a functional fluorescent DHEA analog for live cell imaging.
MedLine Citation:
PMID:  19755137     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The androgen dehydroepiandrosterone (DHEA) has been reported to protect neuronal cells against dysfunction and apoptosis. Several signaling pathways involved in these effects have been described but little is known about the intracellular trafficking of DHEA. We describe design, synthesis and characterization of DHEA-Bodipy, a novel fluorescent DHEA analog. DHEA-Bodipy proved to be a functional DHEA derivative: DHEA-Bodipy (i) induced estrogen receptor alpha-mediated gene activation, (ii) protected PC12 rat pheochromocytoma cells against serum-deprivation-induced apoptosis, and (iii) induced stress fibers and focal adhesion contacts in SH-SY5Y human neuroblastoma cells. DHEA-Bodipy bound rapidly and specifically to plasma membranes of living PC12 cells. We analyzed metabolism and trafficking of DHEA-Bodipy in human neuroblastoma cells. DHEA-Bodipy is the first functional fluorescent DHEA derivative suitable for live cell imaging of intracellular DHEA transport and localization.
Authors:
Susanne Lemcke; Christoph H??nnscheidt; Gustav Waschatko; Anita Bopp; Dieter L??tjohann; Norbert Bertram; Katja Gehrig-Burger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-13
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  314     ISSN:  1872-8057     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-10-27     Completed Date:  2010-01-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  31-40     Citation Subset:  IM    
Affiliation:
Institute of Biochemistry, University of Mainz, Becherweg 30, 55099 Mainz, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / physiology
Biological Transport
Boron Compounds / chemistry*,  metabolism
Cell Membrane / metabolism
Dehydroepiandrosterone / analogs & derivatives*,  metabolism
Estrogen Receptor alpha / metabolism
Fluorescent Dyes / chemistry*,  metabolism
Genes, Reporter
Humans
Microscopy, Fluorescence / methods*
Molecular Structure
Neuroblastoma
PC12 Cells
Rats
Chemical
Reg. No./Substance:
0/4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; 0/Boron Compounds; 0/Estrogen Receptor alpha; 0/Fluorescent Dyes; 53-43-0/Dehydroepiandrosterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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