| DHA-rich phospholipids optimize G-Protein-coupled signaling. | |
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MedLine Citation:
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PMID: 14597917 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To assess the effects of n-3 polyunsaturated phospholipid acyl chains on the initial steps in G-protein-coupled signaling. STUDY DESIGN: Isolated components of the visual signal transduction system, rhodopsin, G protein (G(t)), and phosphodiesterase (PDE), were reconstituted in membranes containing various levels of n-3 polyunsaturated phospholipid acyl chains. In addition, rod outer segment disk membranes containing these components were purified from rats raised on n-3-deficient and n-3-adequate diets. The conformation change of rhodopsin, coupling of rhodopsin to G(t), and PDE activity were each measured separately. RESULTS: The ability of rhodopsin to form the active metarhodopsin II conformation and bind G(t) were both compromised in membranes with reduced levels of n-3 polyunsaturated acyl chains. The activity of PDE, directly related to the integrated cellular response, was reduced in all membranes lacking or deficient in n-3 polyunsaturated acyl chains. PDE activity in membranes containing 22:5n-6 PC was 50% lower than in membranes containing either 22:6n-3 PC or 22:5n-3 PC. CONCLUSIONS: The earliest events in G-protein-coupled signaling; receptor conformation change, receptor-G-protein binding, and PDE activity are reduced in membranes lacking n-3 polyunsaturated acyl chains. Efficient and rapid propagation of G-protein-coupled signaling requires polyunsaturated n-3 phospholipid acyl chains. |
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Authors:
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Drake C Mitchell; Shui-Lin Niu; Burton J Litman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of pediatrics Volume: 143 ISSN: 0022-3476 ISO Abbreviation: J. Pediatr. Publication Date: 2003 Oct |
Date Detail:
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Created Date: 2003-11-04 Completed Date: 2003-12-01 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0375410 Medline TA: J Pediatr Country: United States |
Other Details:
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Languages: eng Pagination: S80-6 Citation Subset: AIM; IM |
Affiliation:
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National Institute on Alcohol Abuse and Alcoholism, Bethesda, Maryland 20892, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Fatty Acids, Omega-3
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physiology* Humans Molecular Conformation Receptors, G-Protein-Coupled / physiology* Retina / physiology Rhodopsin / analogs & derivatives*, physiology* Rod Cell Outer Segment / physiology Signal Transduction / physiology* Structure-Activity Relationship |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Omega-3; 0/Receptors, G-Protein-Coupled; 60383-01-9/metarhodopsins; 9009-81-8/Rhodopsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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