Document Detail

DHA-induced changes of supported lipid membrane morphology.
MedLine Citation:
PMID:  17455967     Owner:  NLM     Status:  MEDLINE    
Docosahexaenoic acid (DHA) is a polyunsaturated long fatty acid known to have fundamental effects on cell membrane function. Here, the effect of DHA on phosphocholine-supported lipid bilayers was measured using the quartz crystal microbalance with dissipation monitoring (QCM-D) technique. Above a concentration of 60 muM (i.e., near the critical micelle concentration), DHA had drastic effects on the viscoelastic properties of the supported membranes, suggesting a more complex process and structure than simple insertion of molecules in the bilayer. Fluorescence microscopy revealed the spontaneous formation of elongated out-growths from the bilayers, which were remarkable for their length ( approximately 100 mum) and extensive coverage of the surface. These results demonstrate the applicability of QCM-D as a method to screen for conditions where membrane remodeling occurs but also that complementary techniques are required to describe in more detail the changes in viscoelastic properties of the membrane. These results are highly relevant for the present rapid development in the field of model lipid membranes aiming toward increased knowledge about processes occurring at biological surfaces.
Dorota Thid; Jason J Benkoski; Sofia Svedhem; Bengt Kasemo; Julie Gold
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-04-25
Journal Detail:
Title:  Langmuir : the ACS journal of surfaces and colloids     Volume:  23     ISSN:  0743-7463     ISO Abbreviation:  Langmuir     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-15     Completed Date:  2007-07-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882736     Medline TA:  Langmuir     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5878-81     Citation Subset:  IM    
Department of Applied Physics, Chalmers University of Technology, 412 96 Göteborg, Sweden.
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MeSH Terms
Biosensing Techniques
Docosahexaenoic Acids / chemistry,  pharmacology*
Lipid Bilayers / chemistry
Membrane Lipids / chemistry*
Microscopy, Fluorescence
Phosphatidylcholines / chemistry
Surface Properties
Reg. No./Substance:
0/Lipid Bilayers; 0/Membrane Lipids; 0/Phosphatidylcholines; 14808-60-7/Quartz; 25167-62-8/Docosahexaenoic Acids; 6753-55-5/1-palmitoyl-2-oleoylphosphatidylcholine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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