| DGGE analysis of buffalo manure eubacteria for hydrogen production: effect of pH, temperature and pretreatments. | |
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MedLine Citation:
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PMID: 23014994 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Buffalo dung is a low-cost substrate with plenty of carbohydrates, an optimal carbon/nitrogen ratio, and a rich microbial flora, and could become a valuable source of biogas. Therefore, in the present study we compared the type and amount of specific eubacteria to the different configurations of pH, temperature and thermal pretreatment after fermentation in batch reactors in order to understand the suitability of buffalo manure for hydrogen production. The phylogenetic structure of the microbial community in fermentation samples was studied using denaturing gradient gel electrophoresis to generate fingerprints of 16S rRNA genes. The sequences analysis revealed abundance of the phyla Bacteroidetes and Firmicutes, and in particular of the order Clostridiales. Very active hydrogen producing bacteria belonging to Clostridium cellulosi species were identified demonstrating the suitability of this substrate to produce hydrogen. Moreover, a large fraction of 16S-rDNA amplicons could not be assigned to lower taxonomic ranks, demonstrating that numerous microorganisms involved in anaerobic fermentation in digesters or bioreactors are still unclassified or unknown. |
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Authors:
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Petronia Carillo; Claudia Carotenuto; Filomena Di Cristofaro; Ioannis Kafantaris; Carmine Lubritto; Mario Minale; Biagio Morrone; Stefania Papa; Pasqualina Woodrow |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-9-27 |
Journal Detail:
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Title: Molecular biology reports Volume: - ISSN: 1573-4978 ISO Abbreviation: Mol. Biol. Rep. Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-9-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0403234 Medline TA: Mol Biol Rep Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Life Sciences, Second University of Naples, via Vivaldi 43, 81100, Caserta, Italy, petronia.carillo@unina2.it. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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