Document Detail


DGGE and T-RFLP analysis of bacterial succession during mushroom compost production and sequence-aided T-RFLP profile of mature compost.
MedLine Citation:
PMID:  18654815     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The amount of button mushroom (Agaricus bisporus) harvested from compost is largely affected by the microbial processes taking place during composting and the microbes inhabiting the mature compost. In this study, the microbial changes during the stages of this specific composting process were monitored, and the dominant bacteria of the mature compost were identified to reveal the microbiological background of the favorable properties of the heat-treated phase II mushroom compost. 16S ribosomal deoxyribonucleic acid (rDNA)-based denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T-RFLP) molecular fingerprinting methods were used to track the succession of microbial communities in summer and winter composting cycles. DNA from individual DGGE bands were reamplified and subjected to sequence analysis. Principal component analysis of fingerprints of the composting processes showed intensive changes in bacterial community during the 22-day procedure. Peak temperature samples grouped together and were dominated by Thermus thermophilus. Mature compost patterns were almost identical by both methods (DGGE, T-RFLP). To get an in-depth analysis of the mature compost bacterial community, the sequence data from cultivation of the bacteria and cloning of environmental 16S rDNA were uniquely coupled with the output of the environmental T-RFLP fingerprints (sequence-aided T-RFLP). This method revealed the dominance of a supposedly cellulose-degrading consortium composed of phylotypes related to Pseudoxanthomonas, Thermobifida, and Thermomonospora.
Authors:
Anna J Székely; Rita Sipos; Brigitta Berta; Balázs Vajna; Csaba Hajdú; Károly Márialigeti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-25
Journal Detail:
Title:  Microbial ecology     Volume:  57     ISSN:  1432-184X     ISO Abbreviation:  Microb. Ecol.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-16     Completed Date:  2009-05-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7500663     Medline TA:  Microb Ecol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  522-33     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Eötvös Loránd University, Budapest, Hungary. mikromara@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Agaricales / metabolism
Bacteria / classification,  genetics*,  isolation & purification
DNA Fingerprinting
DNA, Bacterial / genetics
Ecosystem
Electrophoresis, Gel, Pulsed-Field
Phylogeny
Polymorphism, Restriction Fragment Length*
Principal Component Analysis
RNA, Ribosomal, 16S / genetics
Seasons
Sequence Analysis, DNA
Soil / analysis*
Soil Microbiology*
Temperature
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/RNA, Ribosomal, 16S; 0/Soil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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