Document Detail


DEC1 modulates the circadian phase of clock gene expression.
MedLine Citation:
PMID:  18411297     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
DEC1 suppresses CLOCK/BMAL1-enhanced promoter activity, but its role in the circadian system of mammals remains unclear. Here we examined the effect of Dec1 overexpression or deficiency on circadian gene expression triggered with 50% serum. Overexpression of Dec1 delayed the phase of clock genes such as Dec1, Dec2, Per1, and Dbp that contain E boxes in their regulatory regions, whereas it had little effect on the circadian phase of Per2 and Cry1 carrying CACGTT E' boxes. In contrast, Dec1 deficiency advanced the phase of the E-box-containing clock genes but not that of the E'-box-containing clock genes. Accordingly, DEC1 showed strong binding and transrepression on the E box, but not on the E' box, in chromatin immunoprecipitation, electrophoretic mobility shift, and luciferase reporter assays. Dec1-/- mice showed behavioral rhythms with slightly but significantly longer circadian periods under conditions of constant darkness and faster reentrainment to a 6-h phase-advanced shift of a light-dark cycle. Knockdown of Dec2 with small interfering RNA advanced the phase of Dec1 and Dbp expression, and double knockdown of Dec1 and Dec2 had much stronger effects on the expression of the E-box-containing clock genes. These findings suggest that DEC1, along with DEC2, plays a role in the finer regulation and robustness of the molecular clock.
Authors:
Ayumu Nakashima; Takeshi Kawamoto; Kiyomasa K Honda; Taichi Ueshima; Mitsuhide Noshiro; Tomoyuki Iwata; Katsumi Fujimoto; Hiroshi Kubo; Sato Honma; Noriaki Yorioka; Nobuoki Kohno; Yukio Kato
Related Documents :
18662537 - Sirt1 is a circadian deacetylase for core clock components.
11323677 - Constans mediates between the circadian clock and the control of flowering in arabidopsis.
18786387 - A cyanobacterial circadian clockwork.
20070857 - Transcriptional repressor tieg1 regulates bmal1 gene through gc box and controls circad...
21239457 - Gene expression profiles of raw264.7 macrophages stimulated with preparations of lps di...
17292677 - Genome and transcriptome scale portrait of sigma factors in mycobacterium avium subsp. ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-14
Journal Detail:
Title:  Molecular and cellular biology     Volume:  28     ISSN:  1098-5549     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-02     Completed Date:  2008-07-08     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4080-92     Citation Subset:  IM    
Affiliation:
Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima 734-8553, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
ARNTL Transcription Factors
Animals
Basic Helix-Loop-Helix Transcription Factors / genetics*,  metabolism,  physiology*
CLOCK Proteins
Circadian Rhythm
Female
Gene Expression Regulation*
Homeodomain Proteins / genetics*,  physiology*
Humans
Male
Mice
Mice, Transgenic
NIH 3T3 Cells
Stem Cells / cytology
Trans-Activators / metabolism*
Tumor Suppressor Proteins / genetics*,  physiology*
Chemical
Reg. No./Substance:
0/ARNTL Transcription Factors; 0/ARNTL protein, human; 0/Arntl protein, mouse; 0/Basic Helix-Loop-Helix Transcription Factors; 0/Bhlhb2 protein, mouse; 0/DEC1 protein, human; 0/Homeodomain Proteins; 0/Trans-Activators; 0/Tumor Suppressor Proteins; EC 2.3.1.48/CLOCK Proteins; EC 2.3.1.48/CLOCK protein, human; EC 2.3.1.48/Clock protein, mouse
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The surface-exposed carboxyl region of Mycoplasma pneumoniae elongation factor Tu interacts with fib...
Next Document:  CHIP deficiency decreases longevity, with accelerated aging phenotypes accompanied by altered protei...