| DDX1, DDX21, and DHX36 helicases form a complex with the adaptor molecule TRIF to sense dsRNA in dendritic cells. | |
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MedLine Citation:
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PMID: 21703541 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The innate immune system detects viral infection predominantly by sensing viral nucleic acids. We report the identification of a viral sensor, consisting of RNA helicases DDX1, DDX21, and DHX36, and the adaptor molecule TRIF, by isolation and sequencing of poly I:C-binding proteins in myeloid dendritic cells (mDCs). Knockdown of each helicase or TRIF by shRNA blocked the ability of mDCs to mount type I interferon (IFN) and cytokine responses to poly I:C, influenza A virus, and reovirus. Although DDX1 bound poly I:C via its Helicase A domain, DHX36 and DDX21 bound the TIR domain of TRIF via their HA2-DUF and PRK domains, respectively. This sensor was localized within the cytosol, independent of the endosomes. Thus, the DDX1-DDX21-DHX36 complex represents a dsRNA sensor that uses the TRIF pathway to activate type I IFN responses in the cytosol of mDCs. |
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Authors:
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Zhiqiang Zhang; Taeil Kim; Musheng Bao; Valeria Facchinetti; Sung Yun Jung; Amir Ali Ghaffari; Jun Qin; Genhong Cheng; Yong-Jun Liu |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Immunity Volume: 34 ISSN: 1097-4180 ISO Abbreviation: Immunity Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-06-27 Completed Date: 2011-08-22 Revised Date: 2012-03-14 |
Medline Journal Info:
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Nlm Unique ID: 9432918 Medline TA: Immunity Country: United States |
Other Details:
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Languages: eng Pagination: 866-78 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Immunology, Center for Cancer Immunology Research, University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptor Proteins, Vesicular Transport
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immunology*,
metabolism Animals Cell Line DEAD-box RNA Helicases / genetics, immunology*, metabolism Dendritic Cells / immunology*, metabolism Humans Mice Protein Binding RNA, Double-Stranded / genetics* Signal Transduction |
| Grant Support | |
ID/Acronym/Agency:
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R01 AI056154-08/AI/NIAID NIH HHS; R01 AI069120-07/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Adaptor Proteins, Vesicular Transport; 0/RNA, Double-Stranded; 0/TICAM-1 protein, mouse; EC 2.7.7.-/DDX1 protein, mouse; EC 2.7.7.-/DDX21 protein, mouse; EC 3.6.1.-/DEAD-box RNA Helicases; EC 3.6.1.-/Dhx36 protein, mouse |
| Comments/Corrections | |
Comment In:
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Nat Rev Immunol. 2011 Aug;11(8):504
[PMID:
21738218
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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