Document Detail

DAN directs endolymphatic sac and duct outgrowth in the avian inner ear.
MedLine Citation:
PMID:  14745948     Owner:  NLM     Status:  MEDLINE    
Bone morphogenetic proteins (BMPs) are expressed in the developing vertebrate inner ear and participate in inner ear axial patterning and the development of its sensory epithelium. BMP antagonists, such as noggin, chordin, gremlin, cerberus, and DAN (differential screening-selected gene aberrative in neuroblastoma) inhibit BMP activity and establish morphogenetic gradients during the patterning of many developing tissues and organs. In this study, the role of the BMP antagonist DAN in inner ear development was investigated. DAN-expressing cell pellets were implanted into the otocyst and the periotic mesenchyme to determine the effects of exogenous DAN on otic development. Similar to the effects on the inner ear seen after exposure of otocysts to the BMP4 antagonist noggin, semicircular canals were truncated or eliminated based upon the site of pellet implantation. Unique to the DAN implantations, however, were effects on the developing endolymphatic duct and sac. In DAN-treated inner ears, endolymphatic ducts and sacs were merged with the crus or grew into the superior semicircular canal. Both the canal and endolymphatic duct and sac effects were rescued by joint implantation of BMP4-expressing cells. Electroporation of DAN antisense morpholinos into the epithelium of stage 15-17 otocysts, blocking DAN protein synthesis, resulted in enlarged endolymphatic ducts and sacs as well as smaller semicircular canals in some cases. Taken together, these data suggest a role for DAN both in helping to regulate BMP activity spatially and temporally and in patterning and partitioning of the medial otic tissue between the endolymphatic duct/sac and medially derived inner ear structures.
Lisa M Gerlach-Bank; Amanda R Cleveland; Kate F Barald
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  229     ISSN:  1058-8388     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-01-27     Completed Date:  2004-09-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  219-30     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley-Liss, Inc.
Department of Cell and Developmental Biology, Cell and Molecular Biology Program, University of Michigan, Ann Arbor, Michigan 48109, USA.
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MeSH Terms
Bone Morphogenetic Proteins / antagonists & inhibitors
COS Cells
Chick Embryo
DNA, Complementary / genetics
DNA-Binding Proteins / physiology
Endolymphatic Duct / embryology*,  physiology
Endolymphatic Sac / embryology*,  physiology
Gene Expression Regulation, Developmental / physiology*
Genes, Tumor Suppressor
Hair Cells, Auditory, Inner / embryology,  physiology
Oligodeoxyribonucleotides, Antisense / genetics
Transcription Factors / physiology
Grant Support
Reg. No./Substance:
0/Bone Morphogenetic Proteins; 0/DNA, Complementary; 0/DNA-Binding Proteins; 0/Oligodeoxyribonucleotides, Antisense; 0/Transcription Factors

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