| D609 inhibits the proliferation of neural progenitor cells. | |
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MedLine Citation:
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PMID: 20568314 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We examined the effect of tricyclodecan-9-yl-xanthogenate (D609), a phosphatidylcholine-specific phospholipase C inhibitor, on the proliferation of adult neural progenitor cells in vitro. D609 (100 microM) decreased the proliferation of neural progenitor cells as measured by the proliferation assay, bromodeoxyuridine incorporation, and cell counting without causing cell death. Immunoblot analysis showed a decrease in the expression of cyclin D1 and phosphorylation of retinoblastoma after the incubation of cells with D609. These results suggest that D609 inhibits the proliferation of neural progenitor cells by reducing the cyclin D1 content resulting in decreased phosphorylation of retinoblastoma and subsequent inhibition of cell division. |
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Authors:
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Haviryaji S G Kalluri; Robert J Dempsey |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neuroreport Volume: 21 ISSN: 1473-558X ISO Abbreviation: Neuroreport Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-21 Completed Date: 2010-10-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9100935 Medline TA: Neuroreport Country: England |
Other Details:
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Languages: eng Pagination: 700-3 Citation Subset: IM |
Affiliation:
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Department of Neurological Surgery, University of Wisconsin, Madison, Wisconsin 53792, USA. h.kalluri@neurosurg.wisc.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Stem Cells
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drug effects*,
physiology Animals Bridged Compounds / pharmacology* Bromodeoxyuridine Cell Count Cell Death / drug effects Cell Proliferation / drug effects* Cells, Cultured Cyclin D1 / metabolism Immunoblotting Neurogenesis / drug effects* Neurons / drug effects*, physiology Phosphodiesterase Inhibitors / pharmacology* Phosphorylation Rats Rats, Inbred SHR Retinoblastoma Protein / metabolism Thiones / pharmacology* Type C Phospholipases / antagonists & inhibitors |
| Chemical | |
Reg. No./Substance:
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0/Bridged Compounds; 0/Phosphodiesterase Inhibitors; 0/Retinoblastoma Protein; 0/Thiones; 136601-57-5/Cyclin D1; 59-14-3/Bromodeoxyuridine; 83373-60-8/tricyclodecane-9-yl-xanthogenate; EC 3.1.4.-/Type C Phospholipases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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