Document Detail


D609 inhibits the proliferation of neural progenitor cells.
MedLine Citation:
PMID:  20568314     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We examined the effect of tricyclodecan-9-yl-xanthogenate (D609), a phosphatidylcholine-specific phospholipase C inhibitor, on the proliferation of adult neural progenitor cells in vitro. D609 (100 microM) decreased the proliferation of neural progenitor cells as measured by the proliferation assay, bromodeoxyuridine incorporation, and cell counting without causing cell death. Immunoblot analysis showed a decrease in the expression of cyclin D1 and phosphorylation of retinoblastoma after the incubation of cells with D609. These results suggest that D609 inhibits the proliferation of neural progenitor cells by reducing the cyclin D1 content resulting in decreased phosphorylation of retinoblastoma and subsequent inhibition of cell division.
Authors:
Haviryaji S G Kalluri; Robert J Dempsey
Related Documents :
10328944 - Imaging cells in the developing nervous system with retrovirus expressing modified gree...
18799544 - Dopaminergic neuronal cluster size is determined during early forebrain patterning.
19564764 - In vitro proliferation and osteoblastic phenotype expression of cells derived from huma...
17324604 - Iris pigment epithelial cell transplantation for degenerative retinal diseases.
18279184 - Porphyromonas gingivalis gingipains cause g(1) arrest in osteoblastic/stromal cells.
21240044 - Human disc nucleus properties and vertebral endplate permeability.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroreport     Volume:  21     ISSN:  1473-558X     ISO Abbreviation:  Neuroreport     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-21     Completed Date:  2010-10-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9100935     Medline TA:  Neuroreport     Country:  England    
Other Details:
Languages:  eng     Pagination:  700-3     Citation Subset:  IM    
Affiliation:
Department of Neurological Surgery, University of Wisconsin, Madison, Wisconsin 53792, USA. h.kalluri@neurosurg.wisc.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult Stem Cells / drug effects*,  physiology
Animals
Bridged Compounds / pharmacology*
Bromodeoxyuridine
Cell Count
Cell Death / drug effects
Cell Proliferation / drug effects*
Cells, Cultured
Cyclin D1 / metabolism
Immunoblotting
Neurogenesis / drug effects*
Neurons / drug effects*,  physiology
Phosphodiesterase Inhibitors / pharmacology*
Phosphorylation
Rats
Rats, Inbred SHR
Retinoblastoma Protein / metabolism
Thiones / pharmacology*
Type C Phospholipases / antagonists & inhibitors
Chemical
Reg. No./Substance:
0/Bridged Compounds; 0/Phosphodiesterase Inhibitors; 0/Retinoblastoma Protein; 0/Thiones; 136601-57-5/Cyclin D1; 59-14-3/Bromodeoxyuridine; 83373-60-8/tricyclodecane-9-yl-xanthogenate; EC 3.1.4.-/Type C Phospholipases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Violence against women. Editor's introduction.
Next Document:  Integration of high-throughput genotyping data into pharmacometric analyses using nonlinear mixed ef...