Document Detail


D2-40/podoplanin expression in the human placenta.
MedLine Citation:
PMID:  21095001     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Placental tissue expresses many lymphatic markers. The current study was undertaken to examine if D2-40/podoplanin, a lymphatic endothelial marker, was expressed in the human placenta, and how it is altered developmentally and pathologically. We examined D2-40/podoplanin and VEGFR-3 expressions in placentas from normotensive pregnancies at different gestational ages and in placentas from women with clinically defined preeclampsia. D2-40 expression in systemic lymphatic vessel endothelium served as a positive control. Protein expression for D2-40, VEGFR-3, and β-actin was determined by Western blot in placentas from normotensive (n = 6) and preeclamptic (n = 5) pregnancies. Our results show that D2-40/podoplanin was strongly expressed in the placenta, mainly as a network plexus pattern in the villous stroma throughout gestation. CD31 was limited to villous core fetal vessel endothelium and VEGFR-3 was found in both villous core fetal vessel endothelium and trophoblasts. D2-40/podoplanin expression was significantly decreased, and VEGFR-3 significantly increased in preeclamptic placental tissues compared to normotensive placental controls. Placental villous stroma is a reticular-like structure, and the localization of D2-40 to the stroma suggests that a lymphatic-like conductive network may exist in the human placenta. D2-40/podoplanin is an O-linked sialoglycoprotein. Although little is known regarding biological functions of sialylated glycoproteins within the placenta, placental D2-40/podoplanin may support fetal vessel angiogenesis during placenta development and reduced D2-40/podoplanin expression in preeclamptic placenta may contribute to altered interstitial fluid homeostasis and impaired angiogenesis in this pregnancy disorder.
Authors:
Y Wang; J Sun; Y Gu; S Zhao; L J Groome; J S Alexander
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-11-20
Journal Detail:
Title:  Placenta     Volume:  32     ISSN:  1532-3102     ISO Abbreviation:  Placenta     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-21     Completed Date:  2011-04-01     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  27-32     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / metabolism*
Antibodies, Monoclonal, Murine-Derived
Case-Control Studies
Chorionic Villi / metabolism
Down-Regulation
Female
Humans
Membrane Glycoproteins / analysis,  metabolism*
Placenta / metabolism*,  pathology
Pre-Eclampsia / metabolism,  pathology
Pregnancy
Pregnancy Trimester, First / metabolism
Pregnancy Trimester, Second / metabolism
Stromal Cells / metabolism
Grant Support
ID/Acronym/Agency:
HL36822/HL/NHLBI NIH HHS; HL65997/HL/NHLBI NIH HHS; R01 HD036822/HD/NICHD NIH HHS; R01 HD036822-09/HD/NICHD NIH HHS; R01 HL065997/HL/NHLBI NIH HHS; R01 HL065997-08/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Monoclonal, Murine-Derived; 0/Membrane Glycoproteins; 0/PDPN protein, human; 0/monoclonal antibody D2-40
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  High-throughput generation of hydrogel microbeads with varying elasticity for cell encapsulation.
Next Document:  Exploring chronic myeloid leukemia patients' reasons for not adhering to the oral anticancer drug im...