Document Detail


D-glucose induces microtubular changes in C1300 neuroblastoma cell line through the incorporation of 3-nitro-L-tyrosine into tubulin.
MedLine Citation:
PMID:  18822798     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The microtubular network of neurons is involved in several functions such as formation and tropism of cellular processes, cell division and intracellular transport. A lot of evidences testify that the microtubular network of neurons can be impaired by oxidative stress. A condition of oxidative stress is often possible when D-glucose overloads its metabolic pathway, resulting in an increase in reactive oxygen species and subsequent neurological disorders. The aim of this work was to check in undifferentiated mouse neuroblastoma cells (C1300) the possible oxidative effects of D-glucose on microtubules. Using a concentration of 110mM D-glucose, cell morphology, growth rate, viability and catalase activity were seriously altered. Noteworthy, an increase in 3-nitro-L-tyrosine and a downregulation of tubulins was found in D-glucose-exposed cells, whereas another cytoskeletal proteins, namely actin, did not show any changes. In conclusion, microtubular network can be impaired by D-glucose through specific nitrosative effects, suggesting a possible mechanism at the basis of hyperglycemia-induced neuronal damage.
Authors:
S Gadau; G Lepore; M Zedda; P Manca; V Chisu; V Farina
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives italiennes de biologie     Volume:  146     ISSN:  0003-9829     ISO Abbreviation:  Arch Ital Biol     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-09-30     Completed Date:  2008-11-06     Revised Date:  2009-10-05    
Medline Journal Info:
Nlm Unique ID:  0372441     Medline TA:  Arch Ital Biol     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  107-17     Citation Subset:  IM    
Affiliation:
Department of Animal Biology, University of Sassari, Via Vienna, 2, 07100 Sussari, Italy. sgadau@uniss.it
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
Cell Line, Tumor
Cell Proliferation / drug effects
Cell Survival / drug effects,  physiology
Cytoskeleton / drug effects
Down-Regulation / drug effects,  physiology
Energy Metabolism / drug effects,  physiology
Glucose / pharmacology*
Hyperglycemia / metabolism,  pathology,  physiopathology
Mice
Microtubules / drug effects,  metabolism*,  pathology
Nerve Degeneration / metabolism,  pathology,  physiopathology
Neuroblastoma
Neurons / drug effects,  metabolism*,  pathology
Oxidative Stress / drug effects,  physiology*
Tubulin / metabolism*
Tyrosine / analogs & derivatives*,  metabolism,  pharmacokinetics
Chemical
Reg. No./Substance:
0/Actins; 0/Tubulin; 3604-79-3/3-nitrotyrosine; 50-99-7/Glucose; 55520-40-6/Tyrosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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