| D-glucose induces microtubular changes in C1300 neuroblastoma cell line through the incorporation of 3-nitro-L-tyrosine into tubulin. | |
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MedLine Citation:
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PMID: 18822798 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The microtubular network of neurons is involved in several functions such as formation and tropism of cellular processes, cell division and intracellular transport. A lot of evidences testify that the microtubular network of neurons can be impaired by oxidative stress. A condition of oxidative stress is often possible when D-glucose overloads its metabolic pathway, resulting in an increase in reactive oxygen species and subsequent neurological disorders. The aim of this work was to check in undifferentiated mouse neuroblastoma cells (C1300) the possible oxidative effects of D-glucose on microtubules. Using a concentration of 110mM D-glucose, cell morphology, growth rate, viability and catalase activity were seriously altered. Noteworthy, an increase in 3-nitro-L-tyrosine and a downregulation of tubulins was found in D-glucose-exposed cells, whereas another cytoskeletal proteins, namely actin, did not show any changes. In conclusion, microtubular network can be impaired by D-glucose through specific nitrosative effects, suggesting a possible mechanism at the basis of hyperglycemia-induced neuronal damage. |
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Authors:
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S Gadau; G Lepore; M Zedda; P Manca; V Chisu; V Farina |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Archives italiennes de biologie Volume: 146 ISSN: 0003-9829 ISO Abbreviation: Arch Ital Biol Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-09-30 Completed Date: 2008-11-06 Revised Date: 2009-10-05 |
Medline Journal Info:
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Nlm Unique ID: 0372441 Medline TA: Arch Ital Biol Country: Italy |
Other Details:
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Languages: eng Pagination: 107-17 Citation Subset: IM |
Affiliation:
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Department of Animal Biology, University of Sassari, Via Vienna, 2, 07100 Sussari, Italy. sgadau@uniss.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Animals Cell Line, Tumor Cell Proliferation / drug effects Cell Survival / drug effects, physiology Cytoskeleton / drug effects Down-Regulation / drug effects, physiology Energy Metabolism / drug effects, physiology Glucose / pharmacology* Hyperglycemia / metabolism, pathology, physiopathology Mice Microtubules / drug effects, metabolism*, pathology Nerve Degeneration / metabolism, pathology, physiopathology Neuroblastoma Neurons / drug effects, metabolism*, pathology Oxidative Stress / drug effects, physiology* Tubulin / metabolism* Tyrosine / analogs & derivatives*, metabolism, pharmacokinetics |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Tubulin; 3604-79-3/3-nitrotyrosine; 50-99-7/Glucose; 55520-40-6/Tyrosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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