Document Detail

D-box is required for the degradation of human Shugoshin and chromosome alignment.
MedLine Citation:
PMID:  17448445     Owner:  NLM     Status:  MEDLINE    
Chromosome segregation and proper alignment in mitosis relies on cohesion between sister chromatids and the interaction of the kinetochore with spindle microtubules. Vertebrate Sgo 1 localizes to kinetochores and is required to prevent premature sister centromere separation in mitosis. Sgo 1 is degraded by the anaphase-promoting complex, allowing the separation of sister centromeres in anaphase. However, little is known about the molecular basis of Sgo 1 degradation and its temporal control during mitosis. Here, we show that APC/C targets human Sgo 1 for degradation through a destruction box motif (D-box) in its C-terminus. Mutation in the D-box causes transient metaphase arrest, and mutation in the D-box leads to defects in chromosome alignment and segregation through its effect on the localization of Aurora B and CENP-E. These results provide a link between sister centromere cohesion and bipolar attachment of kinetochores.
Guosheng Fu; Shasha Hua; Tarsha Ward; Xia Ding; Yong Yang; Zhen Guo; Xuebiao Yao
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-04-12
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  357     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-02     Completed Date:  2007-07-12     Revised Date:  2011-07-11    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  672-8     Citation Subset:  IM    
Laboratory of Cellular Dynamics, University of Science & Technology of China, Hefei National Laboratory, Hefei 230027, China.
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MeSH Terms
Amino Acid Sequence
Anaphase / physiology
Binding Sites
Blotting, Western
Cell Cycle / physiology
Cell Cycle Proteins / genetics,  metabolism,  physiology*
Cell Line
Chromosomal Proteins, Non-Histone / metabolism
Chromosome Segregation / physiology*
Green Fluorescent Proteins / genetics,  metabolism
Hela Cells
Microscopy, Confocal
Mitosis / physiology
Protein-Serine-Threonine Kinases / metabolism
Recombinant Fusion Proteins / genetics,  metabolism
Time Factors
Grant Support
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Chromosomal Proteins, Non-Histone; 0/Recombinant Fusion Proteins; 0/SGOL1 protein, human; 0/centromere protein E; 147336-22-9/Green Fluorescent Proteins; EC Kinases; EC kinase

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