| D-amino acids trigger biofilm disassembly. | |
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MedLine Citation:
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PMID: 20431016 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bacteria form communities known as biofilms, which disassemble over time. In our studies outlined here, we found that, before biofilm disassembly, Bacillus subtilis produced a factor that prevented biofilm formation and could break down existing biofilms. The factor was shown to be a mixture of D-leucine, D-methionine, D-tyrosine, and D-tryptophan that could act at nanomolar concentrations. D-amino acid treatment caused the release of amyloid fibers that linked cells in the biofilm together. Mutants able to form biofilms in the presence of D-amino acids contained alterations in a protein (YqxM) required for the formation and anchoring of the fibers to the cell. D-amino acids also prevented biofilm formation by Staphylococcus aureus and Pseudomonas aeruginosa. D-amino acids are produced by many bacteria and, thus, may be a widespread signal for biofilm disassembly. |
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Authors:
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Ilana Kolodkin-Gal; Diego Romero; Shugeng Cao; Jon Clardy; Roberto Kolter; Richard Losick |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Science (New York, N.Y.) Volume: 328 ISSN: 1095-9203 ISO Abbreviation: Science Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-30 Completed Date: 2010-05-11 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 0404511 Medline TA: Science Country: United States |
Other Details:
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Languages: eng Pagination: 627-9 Citation Subset: IM |
Affiliation:
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Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amino Acids / metabolism*, pharmacology Bacillus subtilis / physiology* Bacterial Proteins / chemistry, metabolism Biofilms* / growth & development Cell Wall Culture Media, Conditioned Genes, Bacterial Leucine / metabolism, pharmacology Methionine / metabolism, pharmacology Molecular Sequence Data Mutation Pseudomonas aeruginosa / physiology Staphylococcus aureus / physiology Stereoisomerism Tryptophan / metabolism, pharmacology Tyrosine / metabolism, pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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CA24487/CA/NCI NIH HHS; GM086258/GM/NIGMS NIH HHS; GM18546/GM/NIGMS NIH HHS; GM58213/GM/NIGMS NIH HHS; R01 GM018568-39/GM/NIGMS NIH HHS; R01 GM058213-14/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Bacterial Proteins; 0/Culture Media, Conditioned; 0/TasA protein, Bacillus subtilis; 55520-40-6/Tyrosine; 61-90-5/Leucine; 63-68-3/Methionine; 73-22-3/Tryptophan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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