Document Detail


D-amino acids trigger biofilm disassembly.
MedLine Citation:
PMID:  20431016     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacteria form communities known as biofilms, which disassemble over time. In our studies outlined here, we found that, before biofilm disassembly, Bacillus subtilis produced a factor that prevented biofilm formation and could break down existing biofilms. The factor was shown to be a mixture of D-leucine, D-methionine, D-tyrosine, and D-tryptophan that could act at nanomolar concentrations. D-amino acid treatment caused the release of amyloid fibers that linked cells in the biofilm together. Mutants able to form biofilms in the presence of D-amino acids contained alterations in a protein (YqxM) required for the formation and anchoring of the fibers to the cell. D-amino acids also prevented biofilm formation by Staphylococcus aureus and Pseudomonas aeruginosa. D-amino acids are produced by many bacteria and, thus, may be a widespread signal for biofilm disassembly.
Authors:
Ilana Kolodkin-Gal; Diego Romero; Shugeng Cao; Jon Clardy; Roberto Kolter; Richard Losick
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  328     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-30     Completed Date:  2010-05-11     Revised Date:  2014-01-16    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  627-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amino Acids / metabolism*,  pharmacology
Bacillus subtilis / physiology*
Bacterial Proteins / chemistry,  metabolism
Biofilms* / growth & development
Cell Wall
Culture Media, Conditioned
Genes, Bacterial
Leucine / metabolism,  pharmacology
Methionine / metabolism,  pharmacology
Molecular Sequence Data
Mutation
Pseudomonas aeruginosa / physiology
Staphylococcus aureus / physiology
Stereoisomerism
Tryptophan / metabolism,  pharmacology
Tyrosine / metabolism,  pharmacology
Grant Support
ID/Acronym/Agency:
CA24487/CA/NCI NIH HHS; GM086258/GM/NIGMS NIH HHS; GM18546/GM/NIGMS NIH HHS; GM58213/GM/NIGMS NIH HHS; R01 GM018568-39/GM/NIGMS NIH HHS; R01 GM058213-14/GM/NIGMS NIH HHS; R01 GM086258/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Bacterial Proteins; 0/Culture Media, Conditioned; 0/TasA protein, Bacillus subtilis; 42HK56048U/Tyrosine; 8DUH1N11BX/Tryptophan; AE28F7PNPL/Methionine; GMW67QNF9C/Leucine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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