Document Detail


D-amino-acid oxidase is not present in the mouse liver.
MedLine Citation:
PMID:  9133654     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Since there are conflict reports on the presence of D-amino-acid oxidase in the mouse liver, this problem was examined. D-Amino-acid oxidase activity was not detected in the homogenates of the mouse liver, lung, or heart, whereas it was detected in the homogenates of the mouse kidney and brain. Western blotting showed that a protein which reacted with the antiserum against pig D-amino-acid oxidase was present in the homogenates of the mouse kidney and brain but not in those of the liver or heart. Northern hybridization using a D-amino-acid oxidase cDNA probe detected a hybridizing signal in poly(A)+ RNAs extracted from the mouse kidney and brain but not in those from the liver, heart, or lung. Reverse transcription-polymerase chain reaction using three primer pairs always amplified D-amino-acid oxidase cDNA fragments of expected sizes in the mouse kidney and brain but very rarely did so in the liver, heart, or lung. The results indicate that D-amino-acid oxidase is not present in the mouse liver in a measurable amount.
Authors:
R Konno; M Sasaki; S Asakura; K Fukui; J Enami; A Niwa
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1335     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-05-23     Completed Date:  1997-05-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  173-81     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Dokkyo University School of Medicine, Mibu, Japan. konno@dokkyomed.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Northern
Blotting, Western
Brain / enzymology
D-Amino-Acid Oxidase / analysis*
Kidney / enzymology
Liver / enzymology*
Lung / enzymology
Mice
Mice, Inbred Strains
Myocardium / enzymology
Polymerase Chain Reaction
Chemical
Reg. No./Substance:
EC 1.4.3.3/D-Amino-Acid Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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