Document Detail

D: -Mannitol formation from D: -glucose in a whole-cell biotransformation with recombinant Escherichia coli.
MedLine Citation:
PMID:  15841369     Owner:  NLM     Status:  MEDLINE    
Recently, we reported on the construction of a whole-cell biotransformation system in Escherichia coli for the production of D: -mannitol from D: -fructose. Supplementation of this strain with extracellular glucose isomerase resulted in the formation of 800 mM D: -mannitol from 1,000 mM D: -glucose. Co-expression of the xylA gene of E. coli in the biotransformation strain resulted in a D: -mannitol concentration of 420 mM from 1,000 mM D: -glucose. This is the first example of conversion of D: -glucose to D: -mannitol with direct coupling of a glucose isomerase to the biotransformation system.
Bj?rn Kaup; Stephanie Bringer-Meyer; Hermann Sahm
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-04-20
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  69     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-11-30     Completed Date:  2006-02-03     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  397-403     Citation Subset:  IM    
Karl-Winnacker-Institut, Bioverfahrenstechnik, DECHEMA e.V., Theodor-Heuss-Allee 25, 60486 Frankfurt, Germany.
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MeSH Terms
Aldose-Ketose Isomerases / genetics*,  metabolism
DNA, Bacterial / genetics
Escherichia coli / cytology,  genetics,  metabolism*
Formate Dehydrogenases / genetics,  metabolism
Genetic Engineering / methods
Glucose / metabolism*
Mannitol / metabolism*
Mannitol Dehydrogenases / genetics,  metabolism
Recombinant Proteins / genetics,  metabolism*
Recombination, Genetic*
Reg. No./Substance:
0/DNA, Bacterial; 0/Recombinant Proteins; 50-99-7/Glucose; 69-65-8/Mannitol; EC 1.1.-/Mannitol Dehydrogenases; EC Dehydrogenases; EC 5.3.1/Aldose-Ketose Isomerases; EC isomerase

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