Document Detail

D-Erythroascorbic acid is an important antioxidant molecule in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  10094636     Owner:  NLM     Status:  MEDLINE    
D-Arabinono-1,4-lactone oxidase catalysing the final step of D-erythroascorbic acid biosynthesis was purified from the mitochondrial fraction of Saccharomyces cerevisiae. Based on the amino acid sequence analysis of the enzyme, an unknown open reading frame (ORF), YML086C, was identified as the ALO1 gene encoding the enzyme. The ORF of ALO1 encoded a polypeptide consisting of 526 amino acids with a calculated molecular mass of 59493Da. The deduced amino acid sequence of the enzyme shared 32% and 21% identity with that of L-gulono-1,4-lactone oxidase from rat and L-galactono-1,4-lactone dehydrogenase from cauliflower, respectively, and contained a putative transmembrane segment and a covalent FAD binding site. Blot hybridization analyses showed that a single copy of the gene was present in the yeast genome and that mRNA of the ALO1 gene was 1.8kb in size. In the alo1 mutants, D-erythroascorbic acid and the activity of D-arabinono-1,4-lactone oxidase could not be detected. The intracellular concentration of D-erythroascorbic acid and the enzyme activity increased up to 6.9-fold and 7.3-fold, respectively, in the transformant cells carrying ALO1 in multicopy plasmid. The alo1 mutants showed increased sensitivity towards oxidative stress, but overexpression of ALO1 made the cells more resistant to oxidative stress.
W K Huh; B H Lee; S T Kim; Y R Kim; G E Rhie; Y W Baek; C S Hwang; J S Lee; S O Kang
Related Documents :
17107826 - Direct electrochemistry of glucose oxidase immobilized on a hexagonal mesoporous silica...
6223456 - L-usnate-urease interactions: binding sites for polymerization.
8000856 - Glyoxylic acid production using immobilized glycolate oxidase and catalase.
12162546 - Cloning and expression of fructosyl-amino acid oxidase gene from corynebacterium sp. 2-...
843466 - The effect of normal pregnancy upon the renal handling of uric acid.
3594086 - Uptake inhibitors potentiate gamma-aminobutyric acid-induced contractile responses in t...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular microbiology     Volume:  30     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-03-25     Completed Date:  1999-03-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  895-903     Citation Subset:  IM    
Department of Microbiology, College of Natural Sciences, and Research Center for Molecular Microbiology, Seoul National University, Republic of Korea.
Data Bank Information
Bank Name/Acc. No.:
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acid Sequence
Ascorbic Acid / physiology*
Gene Expression
Genes, Fungal
Hydrogen Peroxide / pharmacology
Molecular Sequence Data
Saccharomyces cerevisiae / drug effects,  enzymology*,  genetics
Sugar Alcohol Dehydrogenases / genetics,  isolation & purification,  physiology*
Reg. No./Substance:
0/Antioxidants; 50-81-7/Ascorbic Acid; 7722-84-1/Hydrogen Peroxide; 89-65-6/isoascorbic acid; EC 1.1.-/Sugar Alcohol Dehydrogenases; EC 1.1.3.-/D-arabinonolactone oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The structure of an ECF-sigma-dependent, light-inducible promoter from the bacterium Myxococcus xant...
Next Document:  Lorazepam for the prevention of recurrent seizures related to alcohol.