Document Detail


D-Erythroascorbic acid is an important antioxidant molecule in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  10094636     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
D-Arabinono-1,4-lactone oxidase catalysing the final step of D-erythroascorbic acid biosynthesis was purified from the mitochondrial fraction of Saccharomyces cerevisiae. Based on the amino acid sequence analysis of the enzyme, an unknown open reading frame (ORF), YML086C, was identified as the ALO1 gene encoding the enzyme. The ORF of ALO1 encoded a polypeptide consisting of 526 amino acids with a calculated molecular mass of 59493Da. The deduced amino acid sequence of the enzyme shared 32% and 21% identity with that of L-gulono-1,4-lactone oxidase from rat and L-galactono-1,4-lactone dehydrogenase from cauliflower, respectively, and contained a putative transmembrane segment and a covalent FAD binding site. Blot hybridization analyses showed that a single copy of the gene was present in the yeast genome and that mRNA of the ALO1 gene was 1.8kb in size. In the alo1 mutants, D-erythroascorbic acid and the activity of D-arabinono-1,4-lactone oxidase could not be detected. The intracellular concentration of D-erythroascorbic acid and the enzyme activity increased up to 6.9-fold and 7.3-fold, respectively, in the transformant cells carrying ALO1 in multicopy plasmid. The alo1 mutants showed increased sensitivity towards oxidative stress, but overexpression of ALO1 made the cells more resistant to oxidative stress.
Authors:
W K Huh; B H Lee; S T Kim; Y R Kim; G E Rhie; Y W Baek; C S Hwang; J S Lee; S O Kang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular microbiology     Volume:  30     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  1998 Nov 
Date Detail:
Created Date:  1999-03-25     Completed Date:  1999-03-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  895-903     Citation Subset:  IM    
Affiliation:
Department of Microbiology, College of Natural Sciences, and Research Center for Molecular Microbiology, Seoul National University, Republic of Korea.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/U40390
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antioxidants*
Ascorbic Acid / physiology*
Gene Expression
Genes, Fungal
Hydrogen Peroxide / pharmacology
Molecular Sequence Data
Rats
Saccharomyces cerevisiae / drug effects,  enzymology*,  genetics
Sugar Alcohol Dehydrogenases / genetics,  isolation & purification,  physiology*
Chemical
Reg. No./Substance:
0/Antioxidants; 50-81-7/Ascorbic Acid; 7722-84-1/Hydrogen Peroxide; 89-65-6/isoascorbic acid; EC 1.1.-/Sugar Alcohol Dehydrogenases; EC 1.1.3.-/D-arabinonolactone oxidase

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