| Cytotoxity of the trans10,cis12 isomer of conjugated linoleic acid on rat hepatoma and its modulation by other fatty acids, tocopherol, and tocotrienol. | |
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MedLine Citation:
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PMID: 16223339 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study was performed to evaluate the isomer-specific cytotoxic effects of conjugated linoleic acid (CLA) on rat hepatoma dRLh-84 cells in vitro. A 10trans,12cis (10t,12c)-CLA showed a strong cytotoxic effect on dRLh-84 cells in culture, whereas no such effect was observed with 9cis,11trans (9c,11t)-CLA or linoleic acid. The optimum concentration for induction of cytotoxicity by 10t,12c-CLA was 5 to 10 microM, but the effect was alleviated at higher concentrations. Coincubation with oleic or palmitoleic acid and 10t,12c-CLA cancelled the cytotoxic effect, but other major saturated or polyunsaturated fatty acids and eraidic acid did not interfere with 10t,12c-CLA-induced cytotoxity. The cytotoxic effect was also alleviated by alpha-tocopherol (alpha-toc) and alpha-tocotrienol but not by any other antioxidant reagent examined. Significant cytotoxicity of 10t,12c-CLA was detected after only a 15-min incubation, and the most noticeable effect was seen after 3 h. After incubation with 10t,12c-CLA at 10 microM, an additional 90 microM of 10t,12c-CLA or 100 microM of alpha-toc was also able to alleviate the cytotoxicity. When cells were treated with 10 microM 10t,12c-CLA for more than 48 h, treatment with additional CLA or alpha-toc could not prevent cell death. |
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Authors:
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Masao Yamasaki; Eri Nishida; Shinsuke Nou; Hirofumi Tachibana; Koji Yamada |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: In vitro cellular & developmental biology. Animal Volume: 41 ISSN: 1071-2690 ISO Abbreviation: In Vitro Cell. Dev. Biol. Anim. Publication Date: 2005 Jul-Aug |
Date Detail:
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Created Date: 2005-10-14 Completed Date: 2005-12-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9418515 Medline TA: In Vitro Cell Dev Biol Anim Country: United States |
Other Details:
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Languages: eng Pagination: 239-44 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Applied BioScience, Faculty of Agriculture, Miyazaki University, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan. myamasaki@cc.miyazaki-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antioxidants / chemistry, metabolism, toxicity Carcinoma, Hepatocellular / metabolism* Cell Line, Tumor / drug effects* Dose-Response Relationship, Drug Fatty Acids* / chemistry, metabolism, toxicity Isomerism Linoleic Acids, Conjugated / chemistry, metabolism*, toxicity Liver Neoplasms, Experimental / metabolism* Rats Tocopherols / chemistry, metabolism, toxicity* Tocotrienols / chemistry, metabolism, toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Fatty Acids; 0/Linoleic Acids, Conjugated; 0/Tocotrienols; 1406-66-2/Tocopherols |
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