Document Detail

Cytotoxity of the trans10,cis12 isomer of conjugated linoleic acid on rat hepatoma and its modulation by other fatty acids, tocopherol, and tocotrienol.
MedLine Citation:
PMID:  16223339     Owner:  NLM     Status:  MEDLINE    
This study was performed to evaluate the isomer-specific cytotoxic effects of conjugated linoleic acid (CLA) on rat hepatoma dRLh-84 cells in vitro. A 10trans,12cis (10t,12c)-CLA showed a strong cytotoxic effect on dRLh-84 cells in culture, whereas no such effect was observed with 9cis,11trans (9c,11t)-CLA or linoleic acid. The optimum concentration for induction of cytotoxicity by 10t,12c-CLA was 5 to 10 microM, but the effect was alleviated at higher concentrations. Coincubation with oleic or palmitoleic acid and 10t,12c-CLA cancelled the cytotoxic effect, but other major saturated or polyunsaturated fatty acids and eraidic acid did not interfere with 10t,12c-CLA-induced cytotoxity. The cytotoxic effect was also alleviated by alpha-tocopherol (alpha-toc) and alpha-tocotrienol but not by any other antioxidant reagent examined. Significant cytotoxicity of 10t,12c-CLA was detected after only a 15-min incubation, and the most noticeable effect was seen after 3 h. After incubation with 10t,12c-CLA at 10 microM, an additional 90 microM of 10t,12c-CLA or 100 microM of alpha-toc was also able to alleviate the cytotoxicity. When cells were treated with 10 microM 10t,12c-CLA for more than 48 h, treatment with additional CLA or alpha-toc could not prevent cell death.
Masao Yamasaki; Eri Nishida; Shinsuke Nou; Hirofumi Tachibana; Koji Yamada
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  41     ISSN:  1071-2690     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:    2005 Jul-Aug
Date Detail:
Created Date:  2005-10-14     Completed Date:  2005-12-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  United States    
Other Details:
Languages:  eng     Pagination:  239-44     Citation Subset:  IM    
Department of Biochemistry and Applied BioScience, Faculty of Agriculture, Miyazaki University, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan.
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MeSH Terms
Antioxidants / chemistry,  metabolism,  toxicity
Carcinoma, Hepatocellular / metabolism*
Cell Line, Tumor / drug effects*
Dose-Response Relationship, Drug
Fatty Acids* / chemistry,  metabolism,  toxicity
Linoleic Acids, Conjugated / chemistry,  metabolism*,  toxicity
Liver Neoplasms, Experimental / metabolism*
Tocopherols / chemistry,  metabolism,  toxicity*
Tocotrienols / chemistry,  metabolism,  toxicity*
Reg. No./Substance:
0/Antioxidants; 0/Fatty Acids; 0/Linoleic Acids, Conjugated; 0/Tocotrienols; 1406-66-2/Tocopherols

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