Document Detail

Cytotoxicity suppression and cellular uptake enhancement of surface modified magnetic nanoparticles.
MedLine Citation:
PMID:  15522758     Owner:  NLM     Status:  MEDLINE    
The aim of this study was to modify the surfaces of superparamagnetic iron oxide nanoparticles (SPION) with pullulan in order to reduce the cytotoxicity and enhance the cellular uptake of the nanoparticles. In this study, we have prepared and characterised the pullulan coated superparamagnetic iron oxide nanoparticles (Pn-SPION) of size around 40-45 nm with magnetite inner core and hydrophilic outer shell of pullulan. We have investigated the effect of cellular uptake of uncoated and Pn-SPION on cell adhesion/viability, cytotoxicity, morphology and cytoskeleton organisation of human fibroblasts. Cell cytotoxicity/adhesion studies of SPIONs on human dermal fibroblasts showed that the particles are toxic and their internalisation resulted in disruption of cytoskeleton organisation of cells. On the other hand, Pn-SPIONs were found to be non-toxic and induced changes in cytoskeleton organisation different from that observed with SPION. Transmission electron microscopy results indicated that the SPION and Pn-SPION were internalised into cells via different mechanisms, thereby suggesting that the particle endocytosis behaviour is dependent on the surface characteristics of the nanoparticles.
Ajay Kumar Gupta; Mona Gupta
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article    
Journal Detail:
Title:  Biomaterials     Volume:  26     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2005 May 
Date Detail:
Created Date:  2004-11-03     Completed Date:  2005-06-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  1565-73     Citation Subset:  IM    
Centre for Cell Engineering, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK.
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MeSH Terms
Apoptosis / drug effects
Cell Line
Cell Proliferation / drug effects
Cell Survival / drug effects
Cells, Cultured
Coated Materials, Biocompatible / adverse effects,  pharmacology
Cytoskeleton / drug effects,  physiology,  ultrastructure
Drug Implants / adverse effects,  pharmacology*
Endocytosis / drug effects,  physiology*
Ferric Compounds / adverse effects,  pharmacology*
Fibroblasts / cytology*,  drug effects,  physiology*
Glucans / chemistry,  pharmacology*
Magnetics / therapeutic use
Materials Testing
Nanotubes / adverse effects*,  chemistry
Reg. No./Substance:
0/Coated Materials, Biocompatible; 0/Drug Implants; 0/Ferric Compounds; 0/Glucans; 1309-37-1/ferric oxide; 9057-02-7/pullulan

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