Document Detail

Cytotoxicity of natural extract from Tegillarca granosa on ovarian cancer cells is mediated by multiple molecules.
MedLine Citation:
PMID:  19796578     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To determine the cellular and molecular mechanism of cytotoxicity induced by Haishengsu (HSS), nature extract from Tegillarca granosa, toward human ovarian cancer cell lines SKOV-3 and OVCAR-3. METHODS: The cytotoxic effects of HSS on two ovarian cancer cell lines were tested by XTT assay. Cell apoptosis and cell cycle arrest induced by HSS were demonstrated by DNA ladder assay and flow cytometric analysis, respectively. RT-PCR or flow cytometric analysis was used to investigate the expression of bcl-2, caspase-3, p53, beta-catenin, E-cadherin, CD24, and CD44. RESULTS: Continuous exposure to HSS for 48 h produced cytotoxic effects on both cell lines in a concentration dependent manner, which was accompanied by apoptosis and cell cycle arrest. Apoptosis associated gene bcl-2 and caspase-3, tumor metastasis associated gene ?-catenin, but not E-cadherin, and CD24, but not CD44, were involved in the effect of growth inhibition induced by HSS. Although p53 mediated apoptosis induced by HSS in OVCAR-3 cells, it was not required in SKOV-3 cells. CONCLUSION: HSS has a potential cytotoxic effect on human ovarian cancer cells, which was mediated by multiple signal molecules including bcl-2, caspase-3, beta-catenin, and CD24. These findings will provide a theoretical basis for HSS's potential clinical application as a novel marine anti-cancer agent.
Ming-Qing Gao; Yan-Tao Han; Li Zhu; Shou-Guo Chen; Zhen-Yu Hong; Chun-Bo Wang
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Publication Detail:
Type:  Journal Article     Date:  2009-10-01
Journal Detail:
Title:  Clinical and investigative medicine. Médecine clinique et experimentale     Volume:  32     ISSN:  1488-2353     ISO Abbreviation:  Clin Invest Med     Publication Date:  2009  
Date Detail:
Created Date:  2009-10-02     Completed Date:  2009-12-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7804071     Medline TA:  Clin Invest Med     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  E368-75     Citation Subset:  IM    
Yonsei University College of Medicine, Seoul, South Korea.
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MeSH Terms
Antigens, CD24 / metabolism
Antigens, CD44 / metabolism
Antineoplastic Agents / chemistry,  pharmacology*,  therapeutic use*
Apoptosis / drug effects
Arcidae / chemistry*
Caspase 3 / metabolism
Cell Cycle / drug effects
Cell Line, Tumor
Flow Cytometry
Gene Expression Regulation, Neoplastic / drug effects
Ovarian Neoplasms / drug therapy
Proto-Oncogene Proteins c-bcl-2 / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tumor Suppressor Protein p53 / metabolism
beta Catenin / metabolism
Reg. No./Substance:
0/Antigens, CD24; 0/Antigens, CD44; 0/Antineoplastic Agents; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tumor Suppressor Protein p53; 0/beta Catenin; EC 3.4.22.-/Caspase 3

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