| Cytotoxicity of natural extract from Tegillarca granosa on ovarian cancer cells is mediated by multiple molecules. | |
| | |
MedLine Citation:
|
PMID: 19796578 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: To determine the cellular and molecular mechanism of cytotoxicity induced by Haishengsu (HSS), nature extract from Tegillarca granosa, toward human ovarian cancer cell lines SKOV-3 and OVCAR-3. METHODS: The cytotoxic effects of HSS on two ovarian cancer cell lines were tested by XTT assay. Cell apoptosis and cell cycle arrest induced by HSS were demonstrated by DNA ladder assay and flow cytometric analysis, respectively. RT-PCR or flow cytometric analysis was used to investigate the expression of bcl-2, caspase-3, p53, beta-catenin, E-cadherin, CD24, and CD44. RESULTS: Continuous exposure to HSS for 48 h produced cytotoxic effects on both cell lines in a concentration dependent manner, which was accompanied by apoptosis and cell cycle arrest. Apoptosis associated gene bcl-2 and caspase-3, tumor metastasis associated gene ?-catenin, but not E-cadherin, and CD24, but not CD44, were involved in the effect of growth inhibition induced by HSS. Although p53 mediated apoptosis induced by HSS in OVCAR-3 cells, it was not required in SKOV-3 cells. CONCLUSION: HSS has a potential cytotoxic effect on human ovarian cancer cells, which was mediated by multiple signal molecules including bcl-2, caspase-3, beta-catenin, and CD24. These findings will provide a theoretical basis for HSS's potential clinical application as a novel marine anti-cancer agent. |
| | |
Authors:
|
Ming-Qing Gao; Yan-Tao Han; Li Zhu; Shou-Guo Chen; Zhen-Yu Hong; Chun-Bo Wang |
Related Documents
:
|
7772478 - Expression of the apoptosis-suppressing protein bcl-2 in non-melanoma skin cancer. 19361538 - Cyanide-induced death of dopaminergic cells is mediated by uncoupling protein-2 up-regu... 14532118 - Bcl-xl/bcl-2 coordinately regulates apoptosis, cell cycle arrest and cell cycle entry. 12408758 - Overexpression of bax up-regulates the sensitivity of hcc-9204 cell apoptosis induced b... 2479378 - A mitogen for schwann cells is derived from myelin basic protein. 2453178 - Furosemide-sensitive sodium and potassium fluxes in human neonatal erythrocytes. |
Publication Detail:
|
Type: Journal Article Date: 2009-10-01 |
Journal Detail:
|
Title: Clinical and investigative medicine. Médecine clinique et experimentale Volume: 32 ISSN: 1488-2353 ISO Abbreviation: Clin Invest Med Publication Date: 2009 |
Date Detail:
|
Created Date: 2009-10-02 Completed Date: 2009-12-14 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7804071 Medline TA: Clin Invest Med Country: Canada |
Other Details:
|
Languages: eng Pagination: E368-75 Citation Subset: IM |
Affiliation:
|
Yonsei University College of Medicine, Seoul, South Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antigens, CD24 / metabolism Antigens, CD44 / metabolism Antineoplastic Agents / chemistry, pharmacology*, therapeutic use* Apoptosis / drug effects Arcidae / chemistry* Caspase 3 / metabolism Cell Cycle / drug effects Cell Line, Tumor Female Flow Cytometry Gene Expression Regulation, Neoplastic / drug effects Humans Ovarian Neoplasms / drug therapy Proto-Oncogene Proteins c-bcl-2 / metabolism Reverse Transcriptase Polymerase Chain Reaction Tumor Suppressor Protein p53 / metabolism beta Catenin / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD24; 0/Antigens, CD44; 0/Antineoplastic Agents; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tumor Suppressor Protein p53; 0/beta Catenin; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cigarette smoking and chronic low back pain in the adult population.
Next Document: Distraction osteogenesis is inhibited by cancer radiotherapy with Co 60.