Document Detail


Cytotoxicity and mutagenicity of coal oils in the CHO/HGPRT assay.
MedLine Citation:
PMID:  6468346     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We used the Chinese hamster ovary cell/hypoxanthine-guanine phosphoribosyl-transferase (CHO/HGPRT) assay to determine the cytotoxicity and mutagenicity of a crude coal oil, the neutral fraction of this crude, and the following three subfractions of the neutral fraction: aliphatic, neutral polar, and a subfraction composed of polycyclic aromatic hydrocarbons plus neutral nitrogen heterocyclics. We also studied the cytotoxicity and mutagenicity of a blend of light and heavy coal-derived fuel oils before and after hydrogenation. All seven mixtures were highly cytotoxic to CHO cells, but the addition of S9 reduced the cytotoxicity. Also, hydrogenation reduced the cytotoxicity of the blend of coal-derived fuel oils. Although highly cytotoxic, none of the seven mixtures induced a clear mutagenic response in the CHO/HGPRT assay. However, previous work has shown that all of the mixtures except the aliphatic subfraction and the blend after hydrogenation are mutagenic in the histidine-reversion assay in Salmonella typhimurium. Based on chemical analyses of the mixtures, the differential sensitivity of Salmonella and CHO cells to nonmutagenic cytotoxins, and studies of the neutral fraction to which additional benzo[a]pyrene had been added, we conclude that the disparity between the results in Salmonella and those obtained in the CHO/HGPRT assay is probably due to the much greater sensitivity of CHO cells (relative to Salmonella) to the cytotoxins in these coal oils. This sensitivity, coupled with the low concentrations of mutagens relative to nonmutagenic cytotoxins in the coal oils, prevents exposure of the cells to concentrations of the mutagens in the mixtures that are high enough to be quantified in the CHO/HGPRT assay.
Authors:
D M DeMarini; P A Brimer; A W Hsie
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Environmental mutagenesis     Volume:  6     ISSN:  0192-2521     ISO Abbreviation:  Environ Mutagen     Publication Date:  1984  
Date Detail:
Created Date:  1984-09-26     Completed Date:  1984-09-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7909737     Medline TA:  Environ Mutagen     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  517-27     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Biotransformation
Cell Line
Cell Survival / drug effects
Clone Cells
Coal Tar / toxicity*
Cricetinae
Cricetulus
Drug Resistance
Female
Hypoxanthine Phosphoribosyltransferase / genetics*
Microsomes, Liver / metabolism
Mutagenicity Tests
Mutagens / toxicity*
Mutation*
Ovary
Rats
Thioguanine / toxicity
Chemical
Reg. No./Substance:
0/Mutagens; 154-42-7/Thioguanine; 8007-45-2/Coal Tar; EC 2.4.2.8/Hypoxanthine Phosphoribosyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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