Document Detail

Cytotoxicity induced by recombinant human tumor necrosis factor-alpha dependent on the types of its receptors on canine cells.
MedLine Citation:
PMID:  9764400     Owner:  NLM     Status:  MEDLINE    
Based on the recent findings that show how recombinant human tumor necrosis factor (rh-TNF)-alpha has potent antitumor activity on human cancer patients when it locally administrated, we have tested the cytotoxicity of rh-TNF-alpha on 3 canine cultured cells: (1) canine kidney carcinoma (CKCa-1), (2) mastocytoma and (3) Mardin Darby canine kidney cells (MDCK). The cell surface expression of TNF-alpha receptors on these canine cells was also determined with anti-human TNF RI and RII polyclonal antibodies. Our data shows that on CKCa-1 which has TNF RI receptors rh-TNF-alpha induced cytotoxicity. By contrast, it exhibited no toxicity on canine mastocytoma which has mainly RII receptors. The data also suggest actinomycin D (ACT-D), an anticancer antibiotic, enhanced the cytotoxicity of rh-TNF-alpha. Combined with ACT-D, rh-TNF-alpha showed the cytotoxicity on MDCK which possessed both TNF RI and RII receptors. The results indicate that the cytotoxicity of rh-TNF-alpha depends on the presence of TNF RI receptors on canine tumor cells.
M Aoki; N Sasaki; K Nomura; H Katamoto; K Kubo; H Kodama; M Mukamoto; T Shimada; F Ohashi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of veterinary medical science / the Japanese Society of Veterinary Science     Volume:  60     ISSN:  0916-7250     ISO Abbreviation:  J. Vet. Med. Sci.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-12-07     Completed Date:  1998-12-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9105360     Medline TA:  J Vet Med Sci     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  889-95     Citation Subset:  IM    
Department of Veterinary Surgery, College of Agriculture, Osaka Prefecture University, Japan.
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MeSH Terms
Cell Line
Cell Survival / drug effects*
Dog Diseases / pathology
Hela Cells
Kidney Neoplasms / pathology,  veterinary
Lymphocytes / cytology,  drug effects
Mast-Cell Sarcoma / pathology,  veterinary
Recombinant Proteins / toxicity*
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha / physiology,  toxicity*
Reg. No./Substance:
0/Recombinant Proteins; 0/Tumor Necrosis Factor-alpha

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