| Cytotoxicity induced by exposure to natural and synthetic tremolite asbestos: An in vitro pilot study. | |
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MedLine Citation:
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PMID: 22578742 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Mineral fibers are potential carcinogens to humans. In order to help clarify the etiology of the pathological effects of asbestos, cellular reactions to natural and synthetic asbestos fibers were compared using a lung alveolar cancer cell line (A549 epithelial cells), considered the first target of inhaled micro-environmental contaminants. Natural asbestos tremolite (NAT) fibers were collected from rocks in NW Italy. Synthetic asbestos tremolite (SAT) was iron-free and therefore considered as standard tremolite. Both fibers, subjected to mineralogical characterization by X-ray powder diffractometry, electron microscopy and energy dispersive spectrometry, fell within the definition of respirable and potentially carcinogenic fibers. Several signs of functional and structural cell damage were found after treatment with both fibers, documented by viability, motility, and morphological perturbations. Phalloidin labeling showed irregular distribution of cytoskeletal F-actin, whereas immunohistochemical investigations showed abnormal expression of VEGF, Cdc42, β-catenin, assessed as risks indicators for cancer development. Both fibers caused significant loss of viability, even compared to UICC crocidolite, but, while SAT fibers exerted a more direct cytotoxic effect, survival of damaged cells expressing high VEGF levels was detected after NAT contact. This in vitro pilot study outlines potential health risks of NAT fibers in vivo related to their iron content, which could trigger signaling networks connected with cell proliferation and neoplastic transformation. |
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Authors:
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Armanda Pugnaloni; Federica Giantomassi; Guendalina Lucarini; Silvana Capella; Andrea Bloise; Roberto Di Primio; Elena Belluso |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-9 |
Journal Detail:
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Title: Acta histochemica Volume: - ISSN: 1618-0372 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-5-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370320 Medline TA: Acta Histochem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012 Elsevier GmbH. All rights reserved. |
Affiliation:
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Dipartimento di Scienze Cliniche e Molecolari-Istologia, Università Politecnica delle Marche, Via Tronto 10/A 60020 Torrette, Ancona, Italy. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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