Document Detail

Cytotoxicity, in vitro models and preliminary in vivo study of dual physical and chemical gels for endovascular embolization of cerebral aneurysms.
MedLine Citation:
PMID:  23359550     Owner:  NLM     Status:  MEDLINE    
We report the evaluation of dual-gelling poly(N-isopropylacrylamide)-based polymer systems as embolic agents for intracranial aneurysms. These hydrogels undergo gelation physically via temperature-responsiveness of poly(NIPAAm) and chemically through a Michael-addition reaction between thiol and vinyl functional groups on the copolymers. Cytotoxicity studies were performed for biocompatibility of the hydrogels. In vitro glass models were utilized to assess injectability and embolization using the gelling systems and an in vivo swine model was used as proof-of-concept for catheter delivery, injection, and occlusion properties of the hydrogels. Rheology creep tests were conducted for determination of viscoelastic behavior, and degradation of the hydrogels was also investigated. Live/dead and proliferation assays indicated good biocompatibility of the hydrogels. In vitro and in vivo assessment demonstrated that the hydrogels were easily delivered via catheters into the aneurysms. Slight recanalization was observed in vivo, with some adhesion of the gels to the balloon catheter seen in vitro. The materials show creep deformation occurring with time; however, the hydrogels did not degrade over the course of 1.5 year. With the possibility to engineer hydrogels bottom-up for particular applications, these studies show properties that need to be optimized for dual-gelling polymer systems to serve as liquid-to-solid embolic agents for aneurysm treatment.
Hanin H Bearat; Mark C Preul; Brent L Vernon
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-01-28
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  101     ISSN:  1552-4965     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2013 Sep 
Date Detail:
Created Date:  2013-07-23     Completed Date:  2013-12-10     Revised Date:  2014-01-22    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2515-25     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
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MeSH Terms
Acrylamides / administration & dosage*,  chemistry
Biocompatible Materials / administration & dosage,  chemistry
Carotid Artery Diseases / therapy
Cell Survival
Disease Models, Animal
Embolization, Therapeutic / methods*
Hydrogels / administration & dosage,  chemistry
Intracranial Aneurysm / therapy*
Materials Testing
Models, Neurological
NIH 3T3 Cells
Polymers / administration & dosage*,  chemistry
Sus scrofa
Reg. No./Substance:
0/Acrylamides; 0/Biocompatible Materials; 0/Hydrogels; 0/Polymers; 25189-55-3/poly-N-isopropylacrylamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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