| Cytotoxicity of flavonoids toward cultured normal human cells. | |
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MedLine Citation:
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PMID: 15684479 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cytotoxicity of flavonoids, including apigenin, eriodictyol, 3-hydroxyflavone, kaempherol, luteolin, naringenin, quercetin, rutin, and taxifolin, toward cultured human normal cells, i.e., human lung embryonic fibroblasts (TIG-1) and human umbilical vein endothelial (HUVE) cells, was examined. When these normal human cells were incubated with each flavonoid in culture medium for 24 h, some of the flavonoids showed considerable cytotoxicity at relatively high concentrations and in a dose-dependent manner. 3-Hydroxyflavone, luteolin, and apigenin were more toxic toward TIG-1 cells than the other flavonoids, and luteolin, 3-hydroxyflavone, and quercetin were more toxic toward HUVE cells. HUVE cells were more vulnerable to flavonoid cytotoxicity than TIG-1 cells. Using 2',7'-dichlorofluorescin diacetate (DCF-DA), the intracellular reactive oxygen species (ROS) level of flavonoid-treated TIG-1 cells was examined. The ROS level increased significantly in the presence of the flavone apigenin or luteolin or the flavonol 3-hydroxyflavone, quercetin, or kaempherol. These results suggest that these flavones and flavonols exert cytotoxicity through increasing intracellular ROS levels. Further, the incorporation of apigenin, 3-hydroxyflavone, luteolin, and quercetin, which are more toxic, into TIG-1 cells during 24-h incubation was examined. These flavonoids were incorporated into them and the order of their incorporation efficiency was similar to that of their cytotoxicity. In conclusion, some flavonoids are cytotoxic at higher concentrations toward human normal cells. Further, it is suggested that they are incorporated into cells, increase intracellular ROS levels, and then exert cytotoxicity. |
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Authors:
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Mitsuyoshi Matsuo; Naoko Sasaki; Kotaro Saga; Takao Kaneko |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Biological & pharmaceutical bulletin Volume: 28 ISSN: 0918-6158 ISO Abbreviation: Biol. Pharm. Bull. Publication Date: 2005 Feb |
Date Detail:
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Created Date: 2005-02-01 Completed Date: 2005-06-03 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9311984 Medline TA: Biol Pharm Bull Country: Japan |
Other Details:
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Languages: eng Pagination: 253-9 Citation Subset: IM |
Affiliation:
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Department of Biology, Faculty of Science, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan. matsuo@konan-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Survival
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drug effects,
physiology Cells, Cultured Dose-Response Relationship, Drug Embryo, Mammalian Endothelial Cells / drug effects*, physiology Fibroblasts / drug effects*, physiology Flavonoids / toxicity* Humans |
| Chemical | |
Reg. No./Substance:
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0/Flavonoids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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