| Cytotoxicity of chlorhexidine on human osteoblastic cells is related to intracellular glutathione levels. | |
| | |
MedLine Citation:
|
PMID: 20518937 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
AIM: To evaluate the mechanisms of cytotoxicity of chlorhexidine (CHX) in human osteoblastic cells in vitro. METHODOLOGY: Cytotoxicity, cell proliferation and collagen synthesis assays were performed to elucidate the toxic effects of CHX on the human osteoblastic cell line U2OS. To determine whether glutathione (GSH) levels were important in the cytotoxicity of CHX, cells were pre-treated with 2-oxothiazolidine-4-carboxylic acid (OTZ) to boost GSH levels or buthionine sulfoximine (BSO) to deplete GSH. RESULTS: CHX demonstrated a cytotoxic effect to U2OS cells in a dose-dependent manner (P < 0.05). The 50% inhibition concentration of CHX was approximately 0.005%. CHX also inhibited cell proliferation and collagen synthesis (P < 0.05). The addition of OTZ acted as a protective effect on the CHX-induced cytotoxicity (P < 0.05). In contrast, the addition of BSO enhanced the CHX-induced cytotoxicity (P < 0.05). CONCLUSIONS: The levels of CHX tested inhibited cell growth, proliferation and collagen synthesis on U2OS cells. CHX has significant potential for periapical toxicity. GSH depletion might be one of the mechanisms underlying CHX cytotoxicity. |
| | |
Authors:
|
T-H Lee; C-C Hu; S-S Lee; M-Y Chou; Y-C Chang |
Related Documents
:
|
15076007 - The cytotoxicity of iodinated radiocontrast agents on renal cells in vitro. 15793557 - Effects of organophosphorous pesticides used in china on various mammalian cells. 19414387 - Comparison of the cytotoxicity of 4 preparations of anti-t-cell globulins in various he... 20638707 - 3-hydroxy-2-methylene-3-(4-nitrophenylpropanenitrile): a new highly active compound aga... 9020357 - Female infertility in mice lacking connexin 37. 21838537 - Mir-15a/16-1 enhances retinoic acid-mediated differentiation of leukemic cells and is u... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: International endodontic journal Volume: 43 ISSN: 1365-2591 ISO Abbreviation: Int Endod J Publication Date: 2010 May |
Date Detail:
|
Created Date: 2010-06-03 Completed Date: 2010-09-28 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8004996 Medline TA: Int Endod J Country: England |
Other Details:
|
Languages: eng Pagination: 430-5 Citation Subset: D |
Affiliation:
|
Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anti-Infective Agents, Local
/
administration & dosage,
toxicity* Antimetabolites / pharmacology Buthionine Sulfoximine / pharmacology Cell Line Cell Proliferation / drug effects Chlorhexidine / administration & dosage, toxicity* Collagen / biosynthesis, drug effects DNA / biosynthesis, drug effects Dose-Response Relationship, Drug Fluorescent Dyes / diagnostic use Glutathione / antagonists & inhibitors, drug effects* Humans Osteoblasts / drug effects* Prodrugs / pharmacology Pyrrolidonecarboxylic Acid / pharmacology Thiazolidines / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Infective Agents, Local; 0/Antimetabolites; 0/Fluorescent Dyes; 0/Prodrugs; 0/Thiazolidines; 19750-45-9/2-oxothiazolidine-4-carboxylic acid; 5072-26-4/Buthionine Sulfoximine; 55-56-1/Chlorhexidine; 70-18-8/Glutathione; 9007-34-5/Collagen; 9007-49-2/DNA; 98-79-3/Pyrrolidonecarboxylic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Vertical fracture resistance of roots after ultrasonic removal of fractured instruments.
Next Document: Fracture resistance of incisor teeth restored using fibre-reinforced posts and threaded metal posts:...