Document Detail


Cytotoxicity of chlorhexidine on human osteoblastic cells is related to intracellular glutathione levels.
MedLine Citation:
PMID:  20518937     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To evaluate the mechanisms of cytotoxicity of chlorhexidine (CHX) in human osteoblastic cells in vitro. METHODOLOGY: Cytotoxicity, cell proliferation and collagen synthesis assays were performed to elucidate the toxic effects of CHX on the human osteoblastic cell line U2OS. To determine whether glutathione (GSH) levels were important in the cytotoxicity of CHX, cells were pre-treated with 2-oxothiazolidine-4-carboxylic acid (OTZ) to boost GSH levels or buthionine sulfoximine (BSO) to deplete GSH. RESULTS: CHX demonstrated a cytotoxic effect to U2OS cells in a dose-dependent manner (P < 0.05). The 50% inhibition concentration of CHX was approximately 0.005%. CHX also inhibited cell proliferation and collagen synthesis (P < 0.05). The addition of OTZ acted as a protective effect on the CHX-induced cytotoxicity (P < 0.05). In contrast, the addition of BSO enhanced the CHX-induced cytotoxicity (P < 0.05). CONCLUSIONS: The levels of CHX tested inhibited cell growth, proliferation and collagen synthesis on U2OS cells. CHX has significant potential for periapical toxicity. GSH depletion might be one of the mechanisms underlying CHX cytotoxicity.
Authors:
T-H Lee; C-C Hu; S-S Lee; M-Y Chou; Y-C Chang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International endodontic journal     Volume:  43     ISSN:  1365-2591     ISO Abbreviation:  Int Endod J     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-06-03     Completed Date:  2010-09-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8004996     Medline TA:  Int Endod J     Country:  England    
Other Details:
Languages:  eng     Pagination:  430-5     Citation Subset:  D    
Affiliation:
Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Anti-Infective Agents, Local / administration & dosage,  toxicity*
Antimetabolites / pharmacology
Buthionine Sulfoximine / pharmacology
Cell Line
Cell Proliferation / drug effects
Chlorhexidine / administration & dosage,  toxicity*
Collagen / biosynthesis,  drug effects
DNA / biosynthesis,  drug effects
Dose-Response Relationship, Drug
Fluorescent Dyes / diagnostic use
Glutathione / antagonists & inhibitors,  drug effects*
Humans
Osteoblasts / drug effects*
Prodrugs / pharmacology
Pyrrolidonecarboxylic Acid / pharmacology
Thiazolidines / pharmacology
Chemical
Reg. No./Substance:
0/Anti-Infective Agents, Local; 0/Antimetabolites; 0/Fluorescent Dyes; 0/Prodrugs; 0/Thiazolidines; 19750-45-9/2-oxothiazolidine-4-carboxylic acid; 5072-26-4/Buthionine Sulfoximine; 55-56-1/Chlorhexidine; 70-18-8/Glutathione; 9007-34-5/Collagen; 9007-49-2/DNA; 98-79-3/Pyrrolidonecarboxylic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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