| Cytotoxicity of atorvastatin and simvastatin on primary rainbow trout (Oncorhynchus mykiss) hepatocytes. | |
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MedLine Citation:
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PMID: 20600806 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Statins are cholesterol-lowering pharmaceuticals and commonly prescribed drugs in European countries. Their discharge into the aquatic environment has increased in the last few years and they are present at detectable levels in most sewage effluents. The aim of the present study was to quantify the cytotoxic effects of acid and lactone forms of two statins, atorvastatin and simvastatin, as well as selected metabolites (ortho- and para-hydroxy atorvastatin acid, ortho-hydroxy atorvastatin lactone, simvastatin hydroxyl carboxylic acid, and 3''hydroxy simvastatin lactone) to hepatocytes from rainbow trout (Oncorhynchus mykiss). Hepatocytes were exposed for 24, 48, and 72 h to different concentrations of each test substance (0.4-400 microM). Cytotoxicity was measured as metabolic inhibition and loss of membrane integrity with the fluorescent probes alamar blue (AB) and 5-carboxyfluorescein diacetate, acetoxymethyl ester (CFDA-AM), respectively. Atorvastatin, simvastatin, and ortho-hydroxy atorvastatin lactone had dose-dependent cytotoxic effects on hepatocytes. Simvastatin was more toxic than atorvastatin and the lactone form more toxic than the acid form. Exposure time affected atorvastatin and ortho-hydroxy atorvastatin lactone but not simvastatin toxicity. |
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Authors:
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Kathrin Sabine Ellesat; Knut-Erik Tollefsen; Anders Asberg; Kevin V Thomas; Ketil Hylland |
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Publication Detail:
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Type: Journal Article Date: 2010-06-17 |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 24 ISSN: 1879-3177 ISO Abbreviation: Toxicol In Vitro Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-31 Completed Date: 2010-12-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 1610-8 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Biology, University of Oslo, Oslo, Norway. kathrin.ellesat@bio.uio.no |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anticholesteremic Agents / toxicity* Cell Survival / drug effects Cells, Cultured Female Hepatocytes / drug effects*, pathology Heptanoic Acids / toxicity* Male Oncorhynchus mykiss / physiology* Pyrroles / toxicity* Simvastatin / toxicity* Water Pollutants, Chemical / toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Anticholesteremic Agents; 0/Heptanoic Acids; 0/Pyrroles; 0/Water Pollutants, Chemical; 110862-48-1/atorvastatin; 79902-63-9/Simvastatin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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