| Cytotoxicity assessment of gliotoxin and penicillic acid in Tetrahymena pyriformis. | |
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MedLine Citation:
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PMID: 16528685 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Various studies have documented the associations between mold exposure and effects on health. Mycotoxins, which occur in spores and mold fragments, can be involved in processes that have pathological effects, such as adynamia of the immune system, recurrent infections of the respiratory tract, or asthma. Using Tetrahymena pyriformis, a single-cell organism well established as a suitable model for human respiratory epithelium-cell functionalities, we investigated dose-response relationships of the mycotoxins gliotoxin and penicillic acid. Our study focused on the viability (cell count, MTT assay), energy levels (adenosine-5'-triphosphate content), energy-providing processes (MTT reduction per cell), and cell respiration (oxygen consumption). Both mycotoxins acted as cytotoxins in a dose-dependent manner. Gliotoxin had a stronger inhibitory effect (EC50 0.38 microM) than did penicillic acid (EC50 343.19 microM). The energy-providing processes were not inhibited or were only weakly inhibited under the influence of gliotoxin, whereas penicillic acid caused stimulation of the physiological parameters. Summarizing the results, it is clear that the two investigated mycotoxins must have different modes of action. They are not only different in the strength of their toxic effects but also in a variety of physiological aspects. In addition, T. pyriformis showed differences in its ability to overcome the negative effects of particular mycotoxin exposures. |
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Authors:
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C Gräbsch; G Wichmann; N Loffhagen; O Herbarth; A Müller |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Environmental toxicology Volume: 21 ISSN: 1520-4081 ISO Abbreviation: Environ. Toxicol. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-03-15 Completed Date: 2006-08-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100885357 Medline TA: Environ Toxicol Country: United States |
Other Details:
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Languages: eng Pagination: 111-7 Citation Subset: IM |
Copyright Information:
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Copyright 2006 Wiley Periodicals, Inc. |
Affiliation:
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UFZ-Centre for Environmental Research, Leipzig-Halle Ltd., Department of Human Exposure Research and Epidemiology, Leipzig, Germany. carolin.graebsch@ufz.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Animals Cell Proliferation / drug effects Cytotoxins / toxicity* Gliotoxin / toxicity* Oxygen / metabolism Penicillic Acid / toxicity* Tetrahymena pyriformis / cytology, drug effects*, physiology Tetrazolium Salts / metabolism Thiazoles / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Cytotoxins; 0/Tetrazolium Salts; 0/Thiazoles; 298-93-1/thiazolyl blue; 56-65-5/Adenosine Triphosphate; 67-99-2/Gliotoxin; 7782-44-7/Oxygen; 90-65-3/Penicillic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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