| Cytotoxicity of advanced glycation endproducts in human micro- and astroglial cell lines depends on the degree of protein glycation. | |
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MedLine Citation:
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PMID: 18825304 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Advanced glycation endproducts (AGEs) arise from the reaction of sugars with side chains and the N-terminus of proteins and are thought to be involved in the pathogenesis of several diseases by inducing oxidative stress, inflammation and cell death presumably mediated through activation of the receptor of AGE (RAGE). To address the question whether the cell damaging effect of AGE depends on the degree of its protein glycation, differential modified AGEs derived from incubating human serum albumin with increasing concentrations of methyl glyoxal were tested on cell viability, reactive oxygen species (ROS) formation, intracellular ATP levels, and activation of caspases 3/7 in two human glial cell lines, which were used as a model for human glia cells. All AGEs tested, regardless of their degree of modification, were found to induce ROS formation in both microglial (CHME-5) and astroglial cells (U373 MG), while only highly modified AGEs were able to decrease the cell viability and to induce apoptosis. This indicates that apoptotic events may be involved in the change of physiological parameters. |
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Authors:
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Katrin Bigl; Frank Gaunitz; Annett Schmitt; Sven Rothemund; Reinhard Schliebs; Gerald Münch; Thomas Arendt |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-30 |
Journal Detail:
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Title: Journal of neural transmission (Vienna, Austria : 1996) Volume: 115 ISSN: 0300-9564 ISO Abbreviation: J Neural Transm Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-10-30 Completed Date: 2009-01-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9702341 Medline TA: J Neural Transm Country: Austria |
Other Details:
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Languages: eng Pagination: 1545-56 Citation Subset: IM |
Affiliation:
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Interdisciplinary Centre of Clinical Research (IZKF), Faculty of Medicine, University of Leipzig, Inselstr. 22, 04103, Leipzig, Germany. katrin.bigl@yahoo.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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metabolism Apoptosis / drug effects Astrocytes / drug effects*, metabolism Caspase 3 / metabolism Caspase 7 / metabolism Cell Line Cell Survival / drug effects Cells, Cultured Glycosylation End Products, Advanced / chemistry*, toxicity* Humans Microglia / drug effects*, metabolism Proteins / chemistry, metabolism Reactive Oxygen Species / metabolism Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| Chemical | |
Reg. No./Substance:
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0/Glycosylation End Products, Advanced; 0/Proteins; 0/Reactive Oxygen Species; 56-65-5/Adenosine Triphosphate; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 7 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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