Document Detail

Cytotoxicity screening of single-walled carbon nanotubes: detection and removal of cytotoxic contaminants from carboxylated carbon nanotubes.
MedLine Citation:
PMID:  21688794     Owner:  NLM     Status:  MEDLINE    
This study compares the cytotoxicity to cultured mammalian cells of nine different single-walled carbon nanotube (SWNT) products synthesized by a variety of methods and obtained from a cross section of vendors. A standard procedure involving sonication and centrifugation in buffered bovine serum albumin was developed to disperse all the SWNTs in a biocompatible solution to facilitate comparisons. The effect of the SWNTs on the proliferative ability of a standard cell line was then assessed. Of the nine different SWNT materials tested, only two were significantly toxic, and both were functionalized by carboxylation from different vendors. This was unexpected because carboxylation makes SWNTs more water-soluble, which would presumably correlate with better biocompatibility. However, additional purification work demonstrated that the toxic material in the carboxylated SWNT preparations could be separated from the SWNTs by filtration. The filtrate that contained the toxic activity also contained abundant small carbon fragments that had Raman signatures characteristic of amorphous carbon species, suggesting a correlation between toxicity and oxidized carbon fragments. The removal of a toxic contaminant associated with carboxylated SWNTs is important in the development of carboxylated SWNTs for pharmacological applications.
Ruhung Wang; Carole Mikoryak; Synyoung Li; David Bushdiecker; Inga H Musselman; Paul Pantano; Rockford K Draper
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-06-30
Journal Detail:
Title:  Molecular pharmaceutics     Volume:  8     ISSN:  1543-8392     ISO Abbreviation:  Mol. Pharm.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-08-01     Completed Date:  2012-01-26     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  101197791     Medline TA:  Mol Pharm     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1351-61     Citation Subset:  IM    
Department of Chemistry, The University of Texas at Dallas, Richardson, Texas 75080, USA.
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MeSH Terms
Cell Line
Cell Proliferation / drug effects*
Microscopy, Atomic Force
Nanotubes, Carbon / adverse effects*
Spectrum Analysis, Raman
Grant Support
1R41ES018002-01/ES/NIEHS NIH HHS; R41 ES018002-01/ES/NIEHS NIH HHS
Reg. No./Substance:
0/Nanotubes, Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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