Document Detail


Cytotoxicity and internalization of polymer hydrogel capsules by mammalian cells.
MedLine Citation:
PMID:  20614935     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polymer hydrogel capsules comprised of poly(methacrylic acid) chains and cross-linked via disulfide linkages were investigated for their cytotoxicity and mechanism of internalization in a variety of mammalian cells. The capsules were internalized by all the tested cell lines which differed in their morphology and function and over short to medium term (24 h) revealed no reduction in viability and metabolic activity of cells. The mechanism of capsule uptake was analyzed using inhibitors of various cellular entry pathways. Of these, blocking the clathrin-mediated endocytotic pathway resulted in a statistically significant reduction in capsule uptake, suggesting this was the predominant pathway of capsule entry in these cell lines. The uptake of solid particles with similar surface chemistry was not significantly decreased by the inhibitor of the clathrin-mediated pathway, which suggested that softness and concomitant flexibility of the hydrogel capsules were factors governing the entry mechanism. This work represents the first systematic study of the interaction of polymer hydrogel capsules with mammalian cells and provides essential information for the application of these capsules in biomedicine.
Authors:
Alexander N Zelikin; Kerry Breheney; Remy Robert; Elvira Tjipto; Kim Wark
Related Documents :
18245255 - Use of drosophila s2 cells as a model for studying ehrlichia chaffeensis infections.
20423125 - A low-cost, rapid deposition method for "smart" films: applications in mammalian cell r...
10821645 - Macrocyclic chelators with paramagnetic cations are internalized into mammalian cells v...
16923165 - Short interfering rna-induced gene silencing is transmitted between cells from the mamm...
19492085 - Mitochondrial alterations in pink1 deficient cells are influenced by calcineurin-depend...
19619455 - A 3d hybrid model for tissue growth: the interplay between cell population and mass tra...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomacromolecules     Volume:  11     ISSN:  1526-4602     ISO Abbreviation:  Biomacromolecules     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-09     Completed Date:  2010-12-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2123-9     Citation Subset:  IM    
Affiliation:
Centre for Nanoscience and Nanotechnology, Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia. zelikin@chem.au.dk
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Endocytosis*
Flow Cytometry
Humans
Hydrogels*
Microscopy, Confocal
Polymers*
Chemical
Reg. No./Substance:
0/Hydrogels; 0/Polymers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Impact of drying on wood ultrastructure: similarities in cell wall alteration between native wood an...
Next Document:  Rational design and simple chemistry yield a superior, neuroprotective HDAC6 inhibitor, tubastatin A...