Document Detail


Cytotoxicity and DNA damage induced by 1, 4-benzoquinone in v79 Chinese hamster lung cells.
MedLine Citation:
PMID:  20391127     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1,4-Benzoquinone (p-BQ) is a metabolite of benzene known to induce genotoxicity in vivo. The aim of this study was to determine whether it also produced genotoxicity in Chinese hamster V79 cells in vitro. In study 1, incubation with p-BQ at different concentrations (25-400 microM) for 2, 12, or 24 h was evaluated by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay to assess cell viability. In study 2, single-cell gel electrophoresis (SCGE) was used to detect DNA damage in V79 cells after exposure to p-BQ at different concentrations (6.25-100 microM). In study 3, reactive oxygen species (ROS) generation was measured using flow cytometry. Data showed that when V79 cells were exposed to 100 microM p-BQ for 2 h, there was a significant increase in cell lethality. DNA damage was detected at concentrations from 6.25 to 100 microM and at time points of 2, 12, and 24 h. ROS production was increased sixfold in BQ-treated (50 microM) cells compared with control. The results of this study suggest that excess production of ROS may be a potential mechanism underlying DNA damage induced by p-BQ.
Authors:
F Yang; J-H Zhou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of toxicology and environmental health. Part A     Volume:  73     ISSN:  1528-7394     ISO Abbreviation:  J. Toxicol. Environ. Health Part A     Publication Date:  2010  
Date Detail:
Created Date:  2010-04-14     Completed Date:  2010-06-17     Revised Date:  2011-02-02    
Medline Journal Info:
Nlm Unique ID:  100960995     Medline TA:  J Toxicol Environ Health A     Country:  England    
Other Details:
Languages:  eng     Pagination:  483-9     Citation Subset:  IM    
Affiliation:
School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Benzoquinones / toxicity*
Cell Line
Cell Survival / drug effects
Comet Assay
Cricetinae
Cricetulus
DNA Damage
Dose-Response Relationship, Drug
Lung / cytology,  drug effects
Mutagens / toxicity*
Reactive Oxygen Species / metabolism
Toxicity Tests
Chemical
Reg. No./Substance:
0/Benzoquinones; 0/Mutagens; 0/Reactive Oxygen Species; 106-51-4/benzoquinone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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