Document Detail

Cytotoxicity of CPT-11 against a Taxol-resistant cell line.
MedLine Citation:
PMID:  9066696     Owner:  NLM     Status:  MEDLINE    
We examined the cytotoxic changes of CPT-11 (SN-38) against a Suit-2 cell line after a long-term culture (more than 4 passages) with Taxol (Taxol-resistant cells). The percentage survival of the Suit-2 cells with and without resistance to 0.8 microM Taxol were 20% and 96%, respectively, after treatment with 0.1 microM Taxol for 24 hours. When Suit-2 cells resistant to 0.6, 0.8, and 1.0 yM of Taxol were treated with 15 pM SN-38 for 2 hours, the percentages of survival were 59%, 72%, and 62%, respectively, and no statistical difference was observed with the percentage survival (77%) of Suit-2 cells cultured without Taxol (parent cells). Moreover, Taxol-resistant cells did not cause an inhibition of the relaxation of DNA by topoisomerase I. Therefore, the cytotoxic level of SN-38 between Suit-2 cells with and without resistance to Taxol was almost equal. The percentage survival of SN-38-resistant Suit-2 cells decreased markedly with the increase in the dose of Taxol, and no statistical difference was observed in comparison to that of the parent Suit-2 cells. These results thus indicated that the cross-resistance to SN-38 and Taxol would therefore be very difficult to induce in Suit-2 cells.
H Matsuoka; K Yano; S Takiguchi; H Wakasugi; H Tomoda
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anticancer research     Volume:  17     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    1997 Jan-Feb
Date Detail:
Created Date:  1997-04-07     Completed Date:  1997-04-07     Revised Date:  2007-09-25    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  467-71     Citation Subset:  IM    
Clinical Research Institute, National Kyushu Cancer Center, Fukuoka, Japan.
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MeSH Terms
Antineoplastic Agents, Phytogenic / pharmacology*
Camptothecin / analogs & derivatives*,  pharmacology
Dose-Response Relationship, Drug
Drug Resistance
Paclitaxel / pharmacology*
Tumor Cells, Cultured
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 100286-90-6/irinotecan; 33069-62-4/Paclitaxel; 7689-03-4/Camptothecin

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