Document Detail

Cytotoxicity and Alkaline Phosphatase Activity Evaluation of EndoSequence Root Repair Material.
MedLine Citation:
PMID:  22794214     Owner:  NLM     Status:  In-Data-Review    
INTRODUCTION: The purpose of this in vitro study was to evaluate the cytotoxicity and alkaline phosphatase (ALP) activity of a new bioceramic root repair material, EndoSequence Root Repair Material (ESRRM; Brasseler USA, Savannah, GA), and to compare these characteristics with those of ProRoot MTA (Dentsply Tulsa Dental, Tulsa, OK) and Geristore (GR; Den-Mat LLC, Santa Maria, CA).
METHODS: Human Saos-2 osteoblast-like cells were exposed to 1-, 3-, and 7-day elutes of the materials (100% and 50% strength) for 24 hours after which the bioactivity and ALP activity of the cells were evaluated using a methylthiazol sulfophenyl (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay and para-Nitrophenylphosphate colorimetric assay, respectively. In the positive control group, Triton X-100 (Boehringer Mannheim Corp, Indianapolis, IN) was used to lyse the cells, representing 100% cytotoxicity, and in the negative control group cells received fresh culture medium only. Data were statistically analyzed using the unpaired t test and 1-way analysis of variance.
RESULTS: The results revealed that the bioactivity of the cells as well as ALP activity were significantly decreased after exposure to ESRRM elutes in almost all time periods, both in 100% and 50% concentrations, with the exception of ALP activity of day 1 elutes of ESRRM at 50% concentration. MTA did not change the bioactivity or ALP activity of the cells. GR elutes of 100% concentration reduced the bioactivity on days 1 and 3, whereas GR elutes of 50% concentration affected the cells only on day 1. None of the GR elutes had any effect on ALP activity of the cells.
CONCLUSIONS: It was concluded that ESRRM elutes of all time periods in general reduced the bioactivity and ALP activity of osteoblast-like cells. GR reduced bioactivity only, whereas MTA had no effect on the cells.
Mahmoud Reza Modareszadeh; Peter M Di Fiore; David A Tipton; Narges Salamat
Related Documents :
22833364 - Wettability influences cell behavior on superhydrophobic surfaces with different topogr...
6332814 - Growth-inhibitory activity of lymphoid cell plasma membranes. ii. partial characterizat...
22594324 - Enhanced viability of corneal epithelial cells for efficient transport/storage using a ...
2169614 - Plasminogen-activator inhibitor type 1 is a potent natural inhibitor of extracellular m...
8184014 - The role of intracellular calcium in the cellular response to ionizing radiation.
23000754 - Deleterious effects of mitochondrial ros generated by killerred photodynamic action in ...
Publication Detail:
Type:  Journal Article     Date:  2012-06-08
Journal Detail:
Title:  Journal of endodontics     Volume:  38     ISSN:  1878-3554     ISO Abbreviation:  J Endod     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1101-5     Citation Subset:  D    
Copyright Information:
Copyright © 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
Private Practice, Dallas, Texas.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Sexual dimorphism in periapical inflammation and bone loss from mitogen-activated protein kinase pho...
Next Document:  Comparison of Heat-testing Methodology.