Document Detail


Cytotoxicity of 12 chemicals of known human and animal skin irritation potential in human keratinocyte cultures.
MedLine Citation:
PMID:  20692982     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
12 chemicals were selected for cytotoxicity testing, based on a report of a collaborative study (EC Project 86E3-063/86-460) that compared in vitro cytotoxicity in animal cell models with animal irritation and non-invasive human in vivo data. Neutral red (NR) and acid phosphatase (AP) activity were chosen as endpoints of cytotoxicity. AP activity has been shown to be a specific indicator of cytotoxicity in keratinocytes. NR(50) values (concentration that produces a 50% reduction in NR uptake compared with controls) and AP((peak)) values (concentration at which a peak activity occurs) were calculated for each chemical. Both in vitro assays exhibited good correlations with the animal irritation data. Poor correlations were obtained for comparison of the cutaneous blood flow volume data. The data presented provide useful information on the relationship between in vivo skin irritation and in vitro experimental models.
Authors:
F M Dickson; J N Lawrence; D J Benford
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  8     ISSN:  0887-2333     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  1994 Aug 
Date Detail:
Created Date:  2010-08-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  661-3     Citation Subset:  -    
Affiliation:
Division of Chemical Safety Evaluation, Robens Institute of Health and Safety, University of Surrey, Guildford, Surrey GU2 5XH, UK.
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