Document Detail

Cytotoxic effects of triterpenoid saponins from Androsace umbellata against multidrug resistance (MDR) and non-MDR cells.
MedLine Citation:
PMID:  20803120     Owner:  NLM     Status:  MEDLINE    
Cytotoxicity-guided fractionation and separation of MeOH extract from Androsace umbellata (Lour.) Merr. led to the isolation of four triterpenoid saponins. Compounds isolated from the n-BuOH soluble fraction were identified as saxifragifolin C (1), A (2), B (3), and D (4) by spectroscopic analysis. Antiproliferative effect of isolated compounds were evaluated by the sulforhodamin B assay against multidrug resistance (MDR; MES-SA/DX5 and HCT15/CLO2) and non-MDR (A549, SK-OV-3, SK-MEL-2, MES-SA, and HCT15) human tumor cell lines. All compounds exhibited strong cytotoxicity against non-MDR human tumor cell lines with IC(50) values of 0.19-2.37 muM. MDR cells and non-MDR cells had similar sensitivity to these compounds, however, MDR cells were highly resistant to doxorubicin. Compounds 1-4 induced an increase in the percentage of Annexin V-binding cells, indicating that 1-4 induced apoptosis in RAW 264.7 cells. Also, the condensation of nuclei, a characteristic morphological change of apoptosis, was observed in RAW 264.7 cells by the treatment with n-BuOH fraction, compounds 3 and 4, respectively.
Jong Hyuk Park; Jong Hwan Kwak; Ja Heouk Khoo; Sung-Hoon Park; Dae Up Kim; Dong Mun Ha; Sang Un Choi; Se Chan Kang; Ok Pyo Zee
Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2010-08-28
Journal Detail:
Title:  Archives of pharmacal research     Volume:  33     ISSN:  0253-6269     ISO Abbreviation:  Arch. Pharm. Res.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-30     Completed Date:  2010-12-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8000036     Medline TA:  Arch Pharm Res     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  1175-80     Citation Subset:  IM    
College of Pharmacy, Sungkyunkwan University, Suwon, 440-746, Korea.
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MeSH Terms
Antineoplastic Agents, Phytogenic / administration & dosage,  isolation & purification,  pharmacology
Apoptosis / drug effects
Cell Line
Cell Line, Tumor
Cell Proliferation / drug effects
Doxorubicin / pharmacology
Drug Resistance, Multiple
Drug Resistance, Neoplasm
Inhibitory Concentration 50
Macrophages / drug effects,  metabolism
Neoplasms / drug therapy*,  pathology
Primulaceae / chemistry*
Saponins / administration & dosage,  isolation & purification,  pharmacology*
Triterpenes / administration & dosage,  isolation & purification,  pharmacology*
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Saponins; 0/Triterpenes; 23214-92-8/Doxorubicin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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