| Cytotoxic effects of different concentrations of a carbamide peroxide bleaching gel on odontoblast-like cells MDPC-23. | |
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MedLine Citation:
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PMID: 19353567 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study evaluated the cytotoxic effects of a carbamide peroxide (CP) bleaching gel at different concentrations on odontoblast-like cells. Immortalized cells of the MDPC-23 cell line (30,000 cells/cm(2)) were incubated for 48 h. The bleaching gel was diluted in DMEM culture medium originating extracts with different CP concentrations. The amount (microg/mL) of hydrogen peroxide (H(2)O(2)) released from each extract was measured by the leukocrystal violet/horseradish peroxidase enzyme assay. Five groups (n = 10) were formed according to the CP concentration in the extracts: G1-DMEM (control); G2-0.0001% CP (0.025 microg/mL H(2)O(2)); G3-0.001% CP (0.43 microg/mL H(2)O(2)); G4-0.01% CP (2.21 microg/mL H(2)O(2)); and G5-0.1% CP (29.74 microg/mL H(2)O(2)). MDPC-23 cells were exposed to the bleaching gel extracts for 60 min and cell metabolism was evaluated by the MTT assay. Data were analyzed statistically by one-way ANOVA and Tukey's test (alpha = 0.05). Cell morphology was examined by scanning electron microscopy. The percentages of viable cells were as follows: G1, 100%; G2, 89.41%; G3, 82.4%; G4, 61.5%; and G5, 23.0%. G2 and G3 did not differ significantly (p > 0.05) from G1. The most severe cytotoxic effects were observed in G3 and G4. In conclusion, even at low concentrations, the CP gel extracts presented cytotoxic effects. This cytotoxicity was dose-dependent, and the 0.1% CP concentration caused the most intense cytopathic effects to the MDPC-23 cells. |
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Authors:
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Adriano Fonseca de Lima; Fernanda Campos Rosetti Lessa; Maria Nadir Gasparoto Mancini; Josimeri Hebling; Carlos Alberto de Souza Costa; Giselle Maria Marchi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biomedical materials research. Part B, Applied biomaterials Volume: 90 ISSN: 1552-4981 ISO Abbreviation: J. Biomed. Mater. Res. Part B Appl. Biomater. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-08 Completed Date: 2009-12-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101234238 Medline TA: J Biomed Mater Res B Appl Biomater Country: United States |
Other Details:
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Languages: eng Pagination: 907-12 Citation Subset: IM |
Affiliation:
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Department of Restorative Denstistry, Piracicaba School of Dentistry, State University of Campinas-UNICAMP, Piracicaba, S??o Paulo, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Dental Enamel / drug effects Dose-Response Relationship, Drug Drug Combinations Gels Humans Hydrogen Peroxide / chemistry Microscopy, Electron, Scanning / methods Odontoblasts / cytology*, drug effects Peroxides / chemistry* Tetrazolium Salts / chemistry Thiazoles / chemistry Time Factors Tooth Bleaching / adverse effects*, methods Tooth Discoloration / prevention & control, therapy Urea / analogs & derivatives*, chemistry |
| Chemical | |
Reg. No./Substance:
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0/Drug Combinations; 0/Gels; 0/Peroxides; 0/Tetrazolium Salts; 0/Thiazoles; 124-43-6/carbamide peroxide; 298-93-1/thiazolyl blue; 57-13-6/Urea; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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