Document Detail

Cytotoxic effects of different concentrations of a carbamide peroxide bleaching gel on odontoblast-like cells MDPC-23.
MedLine Citation:
PMID:  19353567     Owner:  NLM     Status:  MEDLINE    
This study evaluated the cytotoxic effects of a carbamide peroxide (CP) bleaching gel at different concentrations on odontoblast-like cells. Immortalized cells of the MDPC-23 cell line (30,000 cells/cm(2)) were incubated for 48 h. The bleaching gel was diluted in DMEM culture medium originating extracts with different CP concentrations. The amount (microg/mL) of hydrogen peroxide (H(2)O(2)) released from each extract was measured by the leukocrystal violet/horseradish peroxidase enzyme assay. Five groups (n = 10) were formed according to the CP concentration in the extracts: G1-DMEM (control); G2-0.0001% CP (0.025 microg/mL H(2)O(2)); G3-0.001% CP (0.43 microg/mL H(2)O(2)); G4-0.01% CP (2.21 microg/mL H(2)O(2)); and G5-0.1% CP (29.74 microg/mL H(2)O(2)). MDPC-23 cells were exposed to the bleaching gel extracts for 60 min and cell metabolism was evaluated by the MTT assay. Data were analyzed statistically by one-way ANOVA and Tukey's test (alpha = 0.05). Cell morphology was examined by scanning electron microscopy. The percentages of viable cells were as follows: G1, 100%; G2, 89.41%; G3, 82.4%; G4, 61.5%; and G5, 23.0%. G2 and G3 did not differ significantly (p > 0.05) from G1. The most severe cytotoxic effects were observed in G3 and G4. In conclusion, even at low concentrations, the CP gel extracts presented cytotoxic effects. This cytotoxicity was dose-dependent, and the 0.1% CP concentration caused the most intense cytopathic effects to the MDPC-23 cells.
Adriano Fonseca de Lima; Fernanda Campos Rosetti Lessa; Maria Nadir Gasparoto Mancini; Josimeri Hebling; Carlos Alberto de Souza Costa; Giselle Maria Marchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical materials research. Part B, Applied biomaterials     Volume:  90     ISSN:  1552-4981     ISO Abbreviation:  J. Biomed. Mater. Res. Part B Appl. Biomater.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-08     Completed Date:  2009-12-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101234238     Medline TA:  J Biomed Mater Res B Appl Biomater     Country:  United States    
Other Details:
Languages:  eng     Pagination:  907-12     Citation Subset:  IM    
Department of Restorative Denstistry, Piracicaba School of Dentistry, State University of Campinas-UNICAMP, Piracicaba, S??o Paulo, Brazil.
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MeSH Terms
Cell Line
Dental Enamel / drug effects
Dose-Response Relationship, Drug
Drug Combinations
Hydrogen Peroxide / chemistry
Microscopy, Electron, Scanning / methods
Odontoblasts / cytology*,  drug effects
Peroxides / chemistry*
Tetrazolium Salts / chemistry
Thiazoles / chemistry
Time Factors
Tooth Bleaching / adverse effects*,  methods
Tooth Discoloration / prevention & control,  therapy
Urea / analogs & derivatives*,  chemistry
Reg. No./Substance:
0/Drug Combinations; 0/Gels; 0/Peroxides; 0/Tetrazolium Salts; 0/Thiazoles; 124-43-6/carbamide peroxide; 298-93-1/thiazolyl blue; 57-13-6/Urea; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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