Document Detail

Cytotoxic effect of saffron stigma aqueous extract on human transitional cell carcinoma and mouse fibroblast.
MedLine Citation:
PMID:  18825622     Owner:  NLM     Status:  MEDLINE    
INTRODUCTION: Saffron has been suggested to have inhibitory effects on tumoral cells. We evaluated the cytotoxic effect of aqueous extract of saffron on human transitional cell carcinoma (TCC) and mouse non-neoplastic fibroblast cell lines. MATERIALS AND METHODS: Human TCC 5637 cell line and mouse fibroblast cell line (L929) were cultivated and incubated with different concentrations of aqueous extract of saffron stigma (50 microg/mL to 4000 microg/mL). Cytotoxic effect of saffron was evaluated by morphologic observation and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay after 24, 48, 72, and 120 hours in each cell line. RESULTS: After 24 hours, morphological observations showed growth inhibitory effects at saffron extract concentrations higher than 200 microg/mL for L929 cells and at concentrations of 50 microg/mL to 200 microg/mL for the TCC cells. These changes became more prominent after 48 hours. However, significant growth inhibitory effects of the extract were shown at concentrations of 400 microg/mL and 800 microg/mL. Higher concentrations of saffron correlated inversely with cell population of both cell lines. Significant reduction of the survived cells was seen at concentrations of 400 microg/mL and 2000 microg/mL for TCC and L929 cell lines, respectively. After 120 hours, decrease in the percentage of survived cells at higher concentrations of saffron extract was seen in both cell lines. At a concentration of 800 microg/mL, the survived L929 cells plummeted to less than 60% after 120 hours, while no TCC cells survived at this time. No L929 cells survived at 2000 microg/mL. CONCLUSION: Saffron aqueous extract has inhibitory effects on the growth of both TCC 5637 and normal L929 cell lines. This effect is dose dependent.
Behzad Feizzadeh; Jalil Tavakkol Afshari; Hassan Rakhshandeh; Alireza Rahimi; Azam Brook; Hassan Doosti
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Urology journal     Volume:  5     ISSN:  1735-546X     ISO Abbreviation:  -     Publication Date:  2008  
Date Detail:
Created Date:  2008-09-30     Completed Date:  2009-01-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101286676     Medline TA:  Urol J     Country:  Iran    
Other Details:
Languages:  eng     Pagination:  161-7     Citation Subset:  IM    
Department of Urology, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
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MeSH Terms
Carcinoma, Transitional Cell / pathology*
Cell Culture Techniques
Cell Line, Tumor
Cell Proliferation / drug effects*
Cell Survival / drug effects
Fibroblasts / drug effects*
Plant Extracts / pharmacology*
Reg. No./Substance:
0/Plant Extracts

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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