Document Detail

Cytotoxic effect of oyster mushroom Pleurotus ostreatus on human androgen-independent prostate cancer PC-3 cells.
MedLine Citation:
PMID:  16822205     Owner:  NLM     Status:  MEDLINE    
Twenty species of edible mushrooms and three purified mushroom polysaccharides were screened for their antitumor potential on human androgen-independent cancer PC-3 cells. A water-soluble extract (POE) prepared from the fresh oyster mushroom Pleurotus ostreatus produced the most significant cytotoxicity on PC-3 cells among the mushroom species tested. At the same time, POE induced a rapid apoptosis on PC-3 cells detected with annexin V-fluorescein isothiocyanate flow cytometry when the cells were exposed to POE (150 microg/mL) for 2 hours. Induced apoptosis was also confirmed by DNA fragment terminal deoxynucleotidyl transferase-mediated X-dUTP nick end labeling staining while POE (200 microg/mL) was added to PC-3 cells for 6 hours. Both cytotoxicity and induced apoptosis mediated by POE in PC-3 cells are dose-dependent. Interestingly, PC-3 cells appeared to be more sensitive to POE in anchorage-independent growth condition. Tumor colony-forming efficiency was dramatically reduced to 4.5% or 0.5% in POE (60 or 120 microg/mL)-supplemented soft agar medium compared with that of POE-free medium (defined as 100%). Temperature in POE processing plays a decisive role for the cytotoxic activity. Bioactivity of POE was eliminated by exposure to high temperature (80 degrees C) for 2 hours; however, it remained stable at a series temperatures of below 40 degrees C. The active fraction POE-F2 was analyzed and identified by size exclusion of high performance liquid chromatography and the CellTiter 96 AQueous Cell Proliferation Assay (Promega, Madison, WI). Since POE-F2 is also sensitive to heat and has strong 280 nm absorption, the results imply that active compounds recovered from P. ostreatus are water-soluble proteins or polypeptides.
Yu-Huan Gu; Gowsala Sivam
Related Documents :
19351425 - Atr-ftir spectroscopy detects alterations induced by organotin(iv) carboxylates in mcf-...
3561605 - Derivatives of benzo(c)fluorene. xxiv. cytostatic effect of benfluron on human leukemic...
3923925 - Tobramycin resistance of pseudomonas aeruginosa cells growing as a biofilm on urinary c...
9713065 - Effects of single, short-term exposures of human retinal pigment epithelial cells to th...
18599525 - Hes-1 is involved in adaptation of adult human beta-cells to proliferation in vitro.
22367315 - Proteasome inhibitor mg132 induces selective apoptosis in glioblastoma cells through in...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of medicinal food     Volume:  9     ISSN:  1096-620X     ISO Abbreviation:  J Med Food     Publication Date:  2006  
Date Detail:
Created Date:  2006-07-06     Completed Date:  2006-09-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9812512     Medline TA:  J Med Food     Country:  United States    
Other Details:
Languages:  eng     Pagination:  196-204     Citation Subset:  IM    
School of Natural Health Sciences, Bastyr University, Kenmore, Washington, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects*
Cell Line, Tumor
Chemical Fractionation
Chromatography, High Pressure Liquid
Flow Cytometry
In Situ Nick-End Labeling
Pleurotus / chemistry*
Prostatic Neoplasms / pathology*
Grant Support
Reg. No./Substance:
0/Antineoplastic Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Modulatory effects of resveratrol, citroflavan-3-ol, and plant-derived extracts on oxidative stress ...
Next Document:  The antioxidant properties of garlic compounds: allyl cysteine, alliin, allicin, and allyl disulfide...