Document Detail

Cytotoxic effect of achatinin(H) (lectin) from Achatina fulica against a human mammary carcinoma cell line (MCF7).
MedLine Citation:
PMID:  17876678     Owner:  NLM     Status:  MEDLINE    
The hemolymph-derived achatinin(H) (lectin) from Achatina fulica showed a marked cytotoxic effect on MCF7, a human mammary carcinoma cell line. IC(50) values as measured by the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide assay for achatinin(H) ranged from 6 to 10 microg/ml in the MCF7 cells. MCF7 cells showed significant morphological changes leading to cell death. The above cell death was observed after 48 h of treatment with 8 microg/ml when compared to untreated cells. Alterations in the tumor marker enzymes, as well as in antioxidant enzymes, were observed after achatinin(H) treatment. The specificity and purity of the achatinin(H) was confirmed by the Western blot assay. Achatinin(H) binding to MCF7 cells was detected by anti-achatinin(H), and visualization of the achatinin(H) binding sites on confluent MCF7 cells was confirmed by flourescein isothiocyanate conjugated secondary antibody. MCF7-treated cells fluoresced, indicating the presence of achatinin(H) binding sites. Fluorescence-activated cell sorting analysis of the cell cycle showed a significant increase in S-phase in MCF7 cells after 48 h of achatinin(H) treatment. The cells were arrested in G(2)/M phase of the cell cycle after 48 h with significant changes in cell viability. Cellular damage was confirmed by agarose gel electrophoresis with the characteristic appearance of a DNA streak in treated MCF7 cells indicating the ongoing apoptosis.
Indra Dharmu; N Ramamurty; Ramalingam Kannan; Mary Babu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-09-18
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  43     ISSN:  1071-2690     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:    2007 Sep-Oct
Date Detail:
Created Date:  2007-10-11     Completed Date:  2008-04-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  United States    
Other Details:
Languages:  eng     Pagination:  306-14     Citation Subset:  IM    
Biomaterials Division, Central Leather Research Institute, Adyar, Chennai 600 020, Tamil Nadu, India.
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MeSH Terms
Antioxidants / metabolism
Blotting, Western
Breast Neoplasms / pathology*
Cell Count
Cell Cycle / drug effects
Cell Death / drug effects
Cell Line, Tumor
Cell Survival / drug effects
DNA, Neoplasm / isolation & purification
Electrophoresis, Agar Gel
Flow Cytometry
Gastropoda / chemistry*
Lectins / isolation & purification*,  toxicity*
Tumor Markers, Biological / metabolism
Reg. No./Substance:
0/Antioxidants; 0/DNA, Neoplasm; 0/Lectins; 0/Tumor Markers, Biological; 0/achatinin(H)
Erratum In:
In Vitro Cell Dev Biol Anim. 2007 Nov-Dec;43(10):379-81

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